Lee Won-Min, Song Young-Bo, Han Ki-Soo, Sim Wan-Sup, Lee Byung-Hoo
Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 13120 Republic of Korea.
NeoCremar Co., Ltd, Seoul, 05702 Republic of Korea.
Food Sci Biotechnol. 2023 Dec 20;33(9):2255-2260. doi: 10.1007/s10068-023-01474-z. eCollection 2024 Jul.
Oligosaccharides have been widely used as prebiotics in the food industry, however their properties have been examined in vitro, without considering hydrolysis in the human digestive tract, especially in the small intestine. Here, we hypothesized that the prebiotic effects and utilization efficiency of ingested oligosaccharides would be altered in the colon, as their structures are partially hydrolyzed during digestion. Different types of oligosaccharides were partially degraded during simulated digestion, and digestible monosaccharides were released from the initial substrates. The growth of some probiotic strains responded to the presence of digestible/absorbable mono- and disaccharides (components of the prebiotic oligosaccharides), but not to that of the oligosaccharides themselves. These findings regarding oligosaccharide degradation in the gastrointestinal tract can be used to achieve greater experimental accuracy when examining the effects of prebiotics on gut flora via in vitro studies (e.g., on fecal fermentation or microbial growth rates).
The online version contains supplementary material available at 10.1007/s10068-023-01474-z.
低聚糖已在食品工业中广泛用作益生元，然而其特性仅在体外进行了研究，未考虑在人体消化道尤其是小肠中的水解情况。在此，我们假设摄入的低聚糖的益生元效应和利用效率在结肠中会发生改变，因为它们的结构在消化过程中会部分水解。不同类型的低聚糖在模拟消化过程中会部分降解，并且可消化的单糖会从初始底物中释放出来。一些益生菌菌株的生长对可消化/可吸收的单糖和双糖（益生元低聚糖的成分）的存在有反应，但对低聚糖本身没有反应。当通过体外研究（例如粪便发酵或微生物生长速率）研究益生元对肠道菌群的影响时，这些关于胃肠道中低聚糖降解的发现可用于提高实验准确性。
在线版本包含可在10.1007/s10068-023-01474-z获取的补充材料。
