Bioinformatics Unit, IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy.
Department of Translational Stem Cell Biology, Research Institute of the Medical University, Varna, Bulgaria.
Methods Mol Biol. 2024;2846:191-213. doi: 10.1007/978-1-0716-4071-5_13.
Cleavage Under Targets and Tagmentation (CUT&Tag) is a recent methodology used for robust epigenomic profiling that, unlike conventional chromatin immunoprecipitation (ChIP-Seq), requires only a limited amount of cells as starting material. RNA sequencing (RNA-Seq) reveals the presence and quantity of RNA in a biological sample, describing the continuously changing cellular transcriptome. The integrated analysis of transcriptional activity, histone modifications, and chromatin accessibility via CUT&Tag is still in its infancy compared to the well-established ChIP-Seq. This chapter describes a robust bioinformatics methodology and workflow to perform an integrative CUT&Tag/RNA-Seq analysis.
靶向切割和标签技术（CUT&Tag）是一种最近用于强大的表观基因组分析的方法，与传统的染色质免疫沉淀（ChIP-Seq）不同，它只需要有限数量的细胞作为起始材料。RNA 测序（RNA-Seq）揭示了生物样本中 RNA 的存在和数量，描述了不断变化的细胞转录组。与成熟的 ChIP-Seq 相比，通过 CUT&Tag 进行转录活性、组蛋白修饰和染色质可及性的综合分析仍处于起步阶段。本章描述了一种稳健的生物信息学方法和工作流程，用于执行综合的 CUT&Tag/RNA-Seq 分析。
