Naidoo Jerolen, Hurrell Tracey, Scholefield Janine
Bioengineering and Integrated Genomics Group, Future Production: Chemicals Cluster, Council for Scientific and Industrial Research, PO Box 395, Pretoria 0001, South Africa; Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
Bioengineering and Integrated Genomics Group, Future Production: Chemicals Cluster, Council for Scientific and Industrial Research, PO Box 395, Pretoria 0001, South Africa.
Stem Cell Res. 2024 Dec;81:103534. doi: 10.1016/j.scr.2024.103534. Epub 2024 Aug 10.
The lack of equitable representation of African diversity in scientific resources, such as genome-wide association studies and human induced pluripotent stem cell (hiPSC) repositories, has perpetuated inequalities in the advancement of health research. HiPSCs could be transformative in regenerative and precision medicine, therefore, the generation of diverse lines is critical in the establishment of African-relevant preclinical cellular models. HiPSC lines were derived from two healthy donors of Black African ancestry using Sendai virus reprogramming of dermal fibroblasts, and characterised to confirm stemness markers, trilineage differentiation, and genetic integrity. These hiPSCs represent a valuable resource for modelling African relevant disease biology.
在全基因组关联研究和人类诱导多能干细胞（hiPSC）库等科学资源中，非洲多样性缺乏公平代表性，这使得健康研究进展中的不平等现象长期存在。hiPSC在再生医学和精准医学中可能具有变革性，因此，生成多样化的细胞系对于建立与非洲相关的临床前细胞模型至关重要。使用仙台病毒对两名具有黑非洲血统的健康供体的真皮成纤维细胞进行重编程，从而获得hiPSC系，并对其进行表征以确认干性标志物、三系分化和基因完整性。这些hiPSC是用于模拟与非洲相关疾病生物学的宝贵资源。
