Bobe Daija, Kopylov Mykhailo, Miller Jessalyn, Owji Aaron P, Eng Edward T
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.
bioRxiv. 2024 Aug 5:2024.08.05.606612. doi: 10.1101/2024.08.05.606612.
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. We introduce a cryoEM calibration sample that is a mixture of compatible macromolecules that can be used not only for resolution optimization but also provides multiple reference points for evaluating instrument performance, data quality, and image processing workflows in a single experiment. This combined test specimen provides researchers a reference point for validating their cryoEM pipeline, benchmarking their methodologies, and testing new algorithms.
低温电子显微镜（cryoEM）是一种迅速发展的结构生物学方法，已成功揭示生物系统的分子细节。然而，与具有校准标准的成熟生物物理和分析技术不同，cryoEM缺乏全面的生物测试样品。我们引入了一种cryoEM校准样品，它是兼容大分子的混合物，不仅可用于分辨率优化，还能在单个实验中为评估仪器性能、数据质量和图像处理工作流程提供多个参考点。这种组合测试样本为研究人员提供了一个参考点，用于验证他们的cryoEM流程、对其方法进行基准测试以及测试新算法。
