Simons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.
Acta Crystallogr F Struct Biol Commun. 2024 Nov 1;80(Pt 11):320-327. doi: 10.1107/S2053230X24010318. Epub 2024 Oct 31.
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment. This combined test specimen provides researchers with a reference point for validating their cryoEM pipeline, benchmarking their methodologies and testing new algorithms.
低温电子显微镜（cryoEM）是一种快速发展的结构生物学方法，已成功揭示了生物系统的分子细节。然而，与具有校准标准的既定生物物理和分析技术不同，cryoEM 缺乏全面的生物测试样品。在这里，引入了一种由相容大分子混合物组成的 cryoEM 校准样品，它不仅可用于分辨率优化，而且还可以在单个实验中为评估仪器性能、数据质量和图像处理工作流程提供多个参考点。这种组合测试标本为研究人员提供了一个验证其 cryoEM 管道、基准测试方法和测试新算法的参考点。
