Cell Culture Laboratory, Evangelical University of Goiás - UniEVANGELICA, Anapolis, Goias, 75075-580, Brazil.
Departament of Surgery, Faculty of Veterinary Medicine and Animal Science - University of São Paulo, São Paulo, São Paulo, Brazil.
F1000Res. 2024 May 30;13:554. doi: 10.12688/f1000research.147670.1. eCollection 2024.
Chronic respiratory diseases often necessitate lung transplantation due to irreversible damage. Organ engineering offers hope through stem cell-based organ generation. However, the crucial sterilization step in scaffold preparation poses challenges. This study conducted a systematic review of studies that analysed the extracellular matrix (ECM) conditions of decellularised lungs subjected to different sterilisation processes. A search was performed for articles published in the PubMed, Web of Sciences, Scopus, and SciELO databases according to the PRISMA guidelines. Overall, five articles that presented positive results regarding the effectiveness of the sterilisation process were selected, some of which identified functional damage in the ECM. Was possible concluded that regardless of the type of agent used, physical or chemical, all of them demonstrated that sterilisation somehow harms the ECM. An ideal protocol has not been found to be fully effective in the sterilisation of pulmonary scaffolds for use in tissue and/or organ engineering.
慢性呼吸系统疾病常因不可逆损伤而需要进行肺移植。基于干细胞的器官生成为其提供了希望。然而，支架制备中的关键灭菌步骤仍存在挑战。本研究对分析不同灭菌过程下脱细胞肺细胞外基质（ECM）条件的研究进行了系统综述。根据 PRISMA 指南，在 PubMed、Web of Sciences、Scopus 和 SciELO 数据库中搜索了已发表的文章。总体而言，选择了五篇关于灭菌过程有效性的阳性结果的文章，其中一些文章确定了 ECM 中的功能损伤。可以得出结论，无论使用哪种类型的试剂，物理或化学，它们都表明灭菌在某种程度上会损害 ECM。尚未找到一种理想的方案来完全有效地对用于组织和/或器官工程的肺支架进行灭菌。
