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sp. nov.,分离自中国多种经济作物根际。

sp. nov., isolated from the rhizosphere of multiple cash crops in China.

机构信息

State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.

College of Life Science and Technology of Huazhong Agricultural University, Wuhan 430070, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Mar;74(3). doi: 10.1099/ijsem.0.006303.

Abstract

Three bacterial strains, FP250, FP821, and FP53, were isolated from the rhizosphere soil of oilseed rape, licorice, and habanero pepper in Anhui Province, Xinjiang Uygur Autonomous Region, and Jiangsu Province, PR China, respectively. All strains were shown to grow at 4-37 °C and pH 6.0-9.0, and in the presence of 0-4.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences or housekeeping genes (16S rRNA, , , and ) and phylogenomic analysis showed that strains FP250, FP821, and FP53 belong to the genus , and are closely related to DSM 13647, JCM 11938, 11K1, and DSM 13194. The DNA G+C content of strain FP205 was 59.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values of strain FP205 with the most closely related strain were 93.2 % and 51.4 %, respectively, which is well below the threshold for species differentiation. Strain FP205 contained summed feature 3 (C 6 and/or C 7), summed feature 8 (C 7 and/or C 6) as major fatty acids, and diphosphatidylglycerol along with phosphatidylethanolamine and aminophospholipid as major polar lipids. The predominant isoprenoid quinone was ubiquinone-9. Based on these phenotypic, phylogenetic, and chemotaxonomic results, strain FP205 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FP205 (=ACCC 62447=JCM 35687).

摘要

从中国安徽、新疆和江苏的油菜、甘草和哈瓦那胡椒根际土壤中分别分离到三株细菌菌株 FP250、FP821 和 FP53。所有菌株均在 4-37°C 和 pH 6.0-9.0 条件下生长,并能耐受 0-4.0%(w/v)NaCl。基于 16S rRNA 基因序列或管家基因(16S rRNA、、、和)的系统发育分析和系统基因组分析表明,菌株 FP250、FP821 和 FP53 属于 属,与 DSM 13647、JCM 11938、11K1 和 DSM 13194 密切相关。菌株 FP250 的 DNA G+C 含量为 59.8 mol%。菌株 FP205 与最密切相关菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 93.2%和 51.4%,远低于种间差异的阈值。菌株 FP205 含有 sumFeature 3(C 6 和/或 C 7)和 sumFeature 8(C 7 和/或 C 6)作为主要脂肪酸,二磷脂酰甘油与磷脂酰乙醇胺和氨基磷脂作为主要极性脂。主要的异戊二烯醌是泛醌-9。基于这些表型、系统发育和化学分类学结果,菌株 FP205 代表了 属的一个新种,建议命名为 sp. nov.。模式菌株为 FP205(=ACCC 62447=JCM 35687)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a8/10995727/430fd39dd68e/ijsem-74-06303-g001.jpg

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