Gupta Rachna, Singh Anupama, Nema Prabhat K, Roy Tapas, Kumar Sanjay, Kumar Avvaru Praveen
Department of Food Engineering, National Institute of Food Technology, Entrepreneurship and Management (Institute of National Importance, Under MoFPI, Govt. of India), Kundli, District - Sonipat, Haryana 131028, India.
School of Biomedical Sciences, Galgotias University, Greater Noida 203201, India.
ACS Omega. 2024 Aug 2;9(32):34880-34892. doi: 10.1021/acsomega.4c04468. eCollection 2024 Aug 13.
The present investigation has focused on developing an eco-friendly method to extract bioactive compounds from orange peel using natural deep eutectic solvents (NADES). NADES-II composed of choline chloride (ChCl) and ethylene glycol (1:2) and 50% water shows the maximal extraction yield with higher antioxidant activity in terms of DPPH and ABTS scavenging activity with a high total phenolic content (TPC) and total flavonoid content (TFC). The microwave-assisted extraction (MAE) process was optimized using response surface methodology (RSM) and an artificial neural network (ANN). ANN showed a higher value of and lower values of other statistical parameters when compared to RSM. The ideal extraction conditions were optimized as a 13 min rising time, 52 °C temperature, a 21 min holding time, and a 20 mL/g liquid-to-solid ratio. MAE was compared with the conventional heating-stirring extraction (HSE) method using the NADES-II solvent under optimum conditions. The results show that higher extraction yield and antioxidant capacities (DPPH and ABTS), TPC, and TFC can be obtained from orange peel using the MAE process compared to the HSE process. Overall, this study shows an optimiztic approach for the extraction of bioactive compounds from an orange peel using eco-friendly solvents and microwave technology. It also highlights the potential of this approach for valorizing orange peel waste.
本研究聚焦于开发一种环保方法，即使用天然深共晶溶剂（NADES）从橙皮中提取生物活性化合物。由氯化胆碱（ChCl）和乙二醇（1:2）以及50%水组成的NADES-II在DPPH和ABTS清除活性方面表现出最高的提取产率以及更高的抗氧化活性，同时具有较高的总酚含量（TPC）和总黄酮含量（TFC）。采用响应面法（RSM）和人工神经网络（ANN）对微波辅助萃取（MAE）工艺进行了优化。与RSM相比，ANN显示出更高的 值和更低的其他统计参数值。理想的萃取条件优化为升温时间13分钟、温度52°C、保温时间21分钟以及液固比20 mL/g。在最佳条件下，使用NADES-II溶剂将MAE与传统加热搅拌萃取（HSE）方法进行了比较。结果表明，与HSE工艺相比，使用MAE工艺从橙皮中可获得更高的提取产率和抗氧化能力（DPPH和ABTS）、TPC以及TFC。总体而言，本研究展示了一种使用环保溶剂和微波技术从橙皮中提取生物活性化合物的优化方法。它还突出了这种方法在使橙皮废料增值方面的潜力。
