Zima Katarzyna, Khaidakov Barbara, Sochocka Marta, Ochnik Michał, Lemke Krzysztof, Kowalczyk Paulina
AronPharma Ltd. R&D Department, Trzy Lipy Street 3, 80-172, Gdańsk, Poland.
Department of Physiology, Medical University of Gdańsk, Dębinki 1 Street, 80-211, Gdańsk, Poland.
Heliyon. 2024 Aug 2;10(15):e35630. doi: 10.1016/j.heliyon.2024.e35630. eCollection 2024 Aug 15.
Previous studies have highlighted the beneficial properties of plants rich in polyphenols, such as var. Sevast. (LCK), (AM), and (EP). These plants have demonstrated antioxidant, immunomodulatory, and potential antiviral effects. Thus, the objective of this study was to investigate the impact of the ELA blend, a polyphenol-rich blend containing EP, LCK, and AM, on the cellular mechanisms involved in viral infection. To assess the effects of the ELA blend, various experiments were conducted using A549 cells and a mucociliary tissue 3D model called EpiAirway™. Inflammation and oxidative stress induced by LPS were evaluated through measurements of SOD activity, ELISA, and qPCR analysis. Additionally, antiviral assays were performed in a cell-present environment to examine the blend's effectiveness against HCoV-OC43. The results showed that the ELA blend-treated group exhibited reduced expression of , , , , and in both A549 cells and EpiAirway™. Moreover, the blend enhanced the expression of , , , and in A549 cells. The antiviral activity of the ELA blend was also investigated, i.e. its influence on viral replication cycle, to determine the potential as an antiviral preparation. At the highest non-cytotoxic concentration, the ELA blend demonstrated a 87.5 % reduction in viral titer when administered simultaneously with HCoV-OC43. It emphasize potential ability of the preparation to block viral entry to the host cells. At the same time, ELA blend did not express virucidal activity, i.e. inactivation of free viral particles, against HCoV-OC43. In conclusion, ELA blend displayed antiviral activity and exhibited immunomodulatory and antioxidant effects. Based on these findings, it can be concluded that ELA blend has potential for the prevention and treatment of viral infections.
先前的研究强调了富含多酚的植物的有益特性，如 变种Sevast.（LCK）、（AM）和（EP）。这些植物已表现出抗氧化、免疫调节和潜在的抗病毒作用。因此，本研究的目的是调查ELA混合物（一种含有EP、LCK和AM的富含多酚的混合物）对病毒感染所涉及的细胞机制的影响。为了评估ELA混合物的效果，使用A549细胞和一种名为EpiAirway™的黏液纤毛组织3D模型进行了各种实验。通过测量超氧化物歧化酶（SOD）活性、酶联免疫吸附测定（ELISA）和定量聚合酶链反应（qPCR）分析来评估脂多糖（LPS）诱导的炎症和氧化应激。此外，在有细胞存在的环境中进行抗病毒试验，以检查该混合物对人冠状病毒OC43（HCoV-OC43）的有效性。结果表明，ELA混合物处理组在A549细胞和EpiAirway™中均表现出 、 、 、 和 的表达降低。此外，该混合物增强了A549细胞中 、 、 和 的表达。还研究了ELA混合物的抗病毒活性，即其对病毒复制周期的影响，以确定其作为抗病毒制剂的潜力。在最高非细胞毒性浓度下，ELA混合物与HCoV-OC43同时给药时，病毒滴度降低了87.5%。这强调了该制剂阻断病毒进入宿主细胞的潜在能力。同时，ELA混合物对HCoV-OC43不表现出杀病毒活性，即对游离病毒颗粒的灭活作用。总之，ELA混合物显示出抗病毒活性，并表现出免疫调节和抗氧化作用。基于这些发现，可以得出结论，ELA混合物在预防和治疗病毒感染方面具有潜力。
