Sachidananda Roopa, Bhat Vikram Kemmannu, Kurjogi Mahantesh, Vanti Gulam Nabi L, Kayara Madhura
Department of Anaesthesia, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India.
Department of ENT, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India.
Indian J Anaesth. 2024 Aug;68(8):718-724. doi: 10.4103/ija.ija_1181_23. Epub 2024 Jul 2.
Early diagnosis of sepsis is crucial. The primary objective of this study was to explore the role of uncoupling protein 2 (UCP2) in diagnosing sepsis and septic shock.
This prospective observational study was conducted over 19 months. All adult patients aged more than 18 years with a diagnosis of sepsis or septic shock based on quick sequential organ failure assessment (qSOFA) score were enroled. Blood was drawn for procalcitonin (PCT) and UCP2 on days 0, 3, 7 and 28. Blood samples from 50 healthy volunteers were used as controls. An electrochemiluminescence test was done for PCT. A quantitative enzyme-linked immune sorbent assay was used for UCP2. The Chi-square test was used for qualitative variables and the independent -test for quantitative variables. The receiver operator characteristic curve was used to evaluate the diagnostic efficacy of UCP2.
A total of 128 subjects were included in the study. Out of these, 78 patients (qSOFA score ≥2) were subcategorised into the infection group, sepsis or septic shock group based on the PCT levels. The UCP2 levels in the infection, sepsis or septic shock group were significantly higher than in the control group ( > 0.001). The UCP2 levels correlated with PCT on admission, day 3 and day 7.
The UCP2 levels were significantly higher in sepsis and septic shock groups compared to controls and hence could be a potential diagnostic biomarker of sepsis.
脓毒症的早期诊断至关重要。本研究的主要目的是探讨解偶联蛋白2（UCP2）在脓毒症和脓毒性休克诊断中的作用。
这项前瞻性观察性研究持续了19个月。纳入所有年龄超过18岁、根据快速序贯器官衰竭评估（qSOFA）评分诊断为脓毒症或脓毒性休克的成年患者。在第0天、第3天、第7天和第28天采集血液检测降钙素原（PCT）和UCP2。50名健康志愿者的血液样本用作对照。采用电化学发光法检测PCT。采用定量酶联免疫吸附测定法检测UCP2。定性变量采用卡方检验，定量变量采用独立样本t检验。采用受试者工作特征曲线评估UCP2的诊断效能。
本研究共纳入128名受试者。其中，78例患者（qSOFA评分≥2）根据PCT水平被分类到感染组、脓毒症或脓毒性休克组。感染组、脓毒症或脓毒性休克组的UCP2水平显著高于对照组（P<0.001）。入院时、第3天和第7天，UCP2水平与PCT相关。
与对照组相比，脓毒症和脓毒性休克组的UCP2水平显著升高，因此可能是脓毒症的潜在诊断生物标志物。
