Huang Jinbo, Fussenegger Martin
Department of Biosystems Science and Engineering, ETH Zurich, Klingelbergstrasse 48, CH-4056 Basel, Switzerland.
Department of Biosystems Science and Engineering, ETH Zurich, Klingelbergstrasse 48, CH-4056 Basel, Switzerland; Faculty of Science, University of Basel, Klingelbergstrasse 48, CH-4056 Basel, Switzerland.
Trends Biotechnol. 2025 Jan;43(1):16-42. doi: 10.1016/j.tibtech.2024.07.014. Epub 2024 Aug 22.
In recent decades, the field of synthetic biology has witnessed remarkable progress, driving advances in both research and practical applications. One pivotal area of development involves the design of transgene switches capable of precisely regulating specified outputs and controlling cell behaviors in response to physical cues, which encompass light, magnetic fields, temperature, mechanical forces, ultrasound, and electricity. In this review, we delve into the cutting-edge progress made in the field of physically controlled protein expression in engineered mammalian cells, exploring the diverse genetic tools and synthetic strategies available for engineering targeting cells to sense these physical cues and generate the desired outputs accordingly. We discuss the precision and efficiency limitations inherent in these tools, while also highlighting their immense potential for therapeutic applications.
近几十年来，合成生物学领域取得了显著进展，推动了研究和实际应用的进步。一个关键的发展领域涉及转基因开关的设计，这种开关能够精确调节特定输出，并根据包括光、磁场、温度、机械力、超声波和电在内的物理信号控制细胞行为。在这篇综述中，我们深入探讨了工程化哺乳动物细胞中物理控制蛋白质表达领域的前沿进展，探索了多种遗传工具和合成策略，这些工具和策略可用于工程化靶向细胞以感知这些物理信号并相应地产生所需的输出。我们讨论了这些工具固有的精度和效率限制，同时也强调了它们在治疗应用中的巨大潜力。
