Malek T R, Schmidt J A, Shevach E M
J Immunol. 1985 Apr;134(4):2405-13.
The accessory cell requirements for the induction of the IL 2 receptor by the lectin Con A on murine T cell subsets were directly assayed with anti-IL 2 receptor monoclonal antibodies. Substantial levels of IL 2 receptor expression were induced on T lymphocytes of the MHC class I-restricted, suppressor/cytotoxic phenotype (L3T4-, Ly-2+) in the presence and absence of accessory cells. In contrast, high levels of IL 2 receptor expression could only be induced on T cells of the MHC class II-restricted, helper/inducer phenotype (L3T4+, LY-2-) in the presence, but not in the absence, of accessory cells. Ia- cells such as the P388D1 macrophage line or cultured fibroblasts (DAP X 3) were as efficient as the Ia+ B cell hybridoma LB in providing accessory cell function for the L3T4+, Ly-2- subset. PMA, but not purified human IL 1, could substitute for accessory cells for both IL 2 receptor expression and IL 2 secretion by the L3T4+, Ly-2- subset. These data suggest that IL 2 receptor induction on the L3T4+, Ly-2- subset is complex, possibly requiring a T cell-accessory cell interaction, whereas the lectin may directly trigger IL 2 receptor expression on L3T4-, Ly-2+ T cells.
用抗白细胞介素2（IL-2）受体单克隆抗体直接检测了凝集素刀豆蛋白A（Con A）诱导小鼠T细胞亚群表达IL-2受体时对辅助细胞的需求。在有或没有辅助细胞存在的情况下，主要组织相容性复合体（MHC）I类限制性、抑制/细胞毒性表型（L3T4-，Ly-2+）的T淋巴细胞上均可诱导出相当水平的IL-2受体表达。相比之下，只有在有辅助细胞存在而不是没有辅助细胞时，才能在MHC II类限制性、辅助/诱导表型（L3T4+，LY-2-）的T细胞上诱导出高水平的IL-2受体表达。诸如P388D1巨噬细胞系或培养的成纤维细胞（DAP X 3）等Ia-细胞在为L3T4+，Ly-2-亚群提供辅助细胞功能方面与Ia+B细胞杂交瘤LB一样有效。佛波酯（PMA）而非纯化的人白细胞介素1（IL-1）可替代辅助细胞，促进L3T4+，Ly-2-亚群表达IL-2受体和分泌IL-2。这些数据表明，L3T4+，Ly-2-亚群上IL-2受体的诱导过程较为复杂，可能需要T细胞与辅助细胞相互作用，而凝集素可能直接触发L3T4-，Ly-2+ T细胞上IL-2受体的表达。
