Liu Yixi, Young Kelly, Rakotondraibe L Harinantenaina, Brodie Peggy J, Wiley Jessica D, Cassera Maria B, Callmander Martin W, Rakotondrajaona R, Rakotobe Etienne, Rasamison Vincent E, TenDyke Karen, Shen Yongchun, Kingston David G I
§Missouri Botanical Garden, P.O. Box 299, St. Louis, Missouri 63166, United States.
⊥Centre National d'Application des Recherches Pharmaceutiques, B.P. 702, Antananarivo 101, Madagascar.
J Nat Prod. 2015 Jul 24;78(7):1543-7. doi: 10.1021/np501020m. Epub 2015 Jun 19.
The two new lignans 3α-O-(β-D-glucopyranosyl)desoxypodophyllotoxin (1) and 4-O-(β-D-glucopyranosyl)dehydropodophyllotoxin (2) were isolated from Cleistanthus boivinianus, together with the known lignans deoxypicropodophyllotoxin (3), (±)-β-apopicropodophyllin (4), (-)-desoxypodophyllotoxin (5), (-)-yatein (6), and β-peltatin-5-O-β-D-glucopyranoside (7). The structures of all compounds were characterized by spectroscopic techniques. Compounds 1, 4, and 5 showed potent antiproliferative activities against the A2780 ovarian cancer cell line, with IC50 values of 33.0 ± 3.6, 63.1 ± 6.7, and 230 ± 1 nM, respectively. Compounds 2 and 7 showed only modest A2780 activities, with IC50 values of 2.1 ± 0.3 and 4.9 ± 0.1 μM, respectively, while compounds 3 and 6 had IC50 values of >10 μM. Compound 1 also had potent antiproliferative activity against the HCT-116 human colon carcinoma cell line, with an IC50 value of 20.5 nM, and compound 4 exhibited modest antiproliferative activity against the A2058 human caucasian metastatic melanoma and MES-SA human uterine sarcoma cell lines, with IC50 values of 4.6 and 4.0 μM, respectively.
从布氏闭花木（Cleistanthus boivinianus）中分离出两种新的木脂素3α - O -（β - D - 吡喃葡萄糖基）脱氧鬼臼毒素（1）和4 - O -（β - D - 吡喃葡萄糖基）脱氢鬼臼毒素（2），以及已知的木脂素脱氧苦鬼臼毒素（3）、（±）-β - 阿朴苦鬼臼脂素（4）、（-）-脱氧鬼臼毒素（5）、（-）-叶下珠素（6）和β - 盾叶鬼臼毒素 - 5 - O - β - D - 吡喃葡萄糖苷（7）。所有化合物的结构均通过光谱技术进行了表征。化合物1、4和5对A2780卵巢癌细胞系显示出较强的抗增殖活性，IC50值分别为33.0±3.6、63.1±6.7和230±1 nM。化合物2和7对A2780细胞系仅表现出适度的活性，IC50值分别为2.1±0.3和4.9±0.1 μM，而化合物3和6的IC50值>10 μM。化合物1对HCT - 116人结肠癌细胞系也具有较强的抗增殖活性，IC50值为20.5 nM，化合物4对A2058人白种人转移性黑色素瘤和MES - SA人子宫肉瘤细胞系表现出适度的抗增殖活性，IC50值分别为4.6和4.0 μM。
