Tain You-Lin, Hou Chih-Yao, Chang-Chien Guo-Ping, Lin Shu-Fen, Hsu Chien-Ning
Division of Pediatric Nephrology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan.
College of Medicine, Chang Gung University, Taoyuan 330, Taiwan.
Antioxidants (Basel). 2024 Aug 2;13(8):944. doi: 10.3390/antiox13080944.
Pregnant women with chronic kidney disease (CKD) face increased risks of adverse outcomes in their adult offspring. Offspring rats born to dams fed an adenine diet develop hypertension, coinciding with dysregulated hydrogen sulfide (HS) and nitric oxide (NO) pathways, as well as alterations in gut microbiota. Chondroitin sulfate (CS) is a multifunctional food known for its diverse bioactivities. As a sulfate prebiotic, CS has shown therapeutic potential in various diseases. Here, we investigated the protective effects of maternal CS supplementation against hypertension in offspring induced by an adenine diet. Mother rats were administered regular chow, 0.5% adenine, 3% CS, or a combination throughout gestation and lactation. Maternal CS supplementation effectively protected offspring from hypertension induced by the adenine diet. These beneficial effects of CS were connected with increased renal mRNA and protein levels of 3-mercaptopyruvate sulfurtransferase, an enzyme involved in HS production. Furthermore, maternal CS treatment significantly enhanced alpha diversity and altered beta diversity of gut microbiota in adult offspring. Specifically, perinatal CS treatment promoted the abundance of beneficial microbes such as and . In conclusion, perinatal CS treatment mitigates offspring hypertension associated with maternal adenine diet, suggesting that early administration of sulfate prebiotics may hold preventive potential. These findings warrant further translational research to explore their clinical implications.
患有慢性肾脏病（CKD）的孕妇其成年后代面临不良结局的风险增加。喂食腺嘌呤饮食的母鼠所生的后代大鼠会出现高血压，这与硫化氢（HS）和一氧化氮（NO）通路失调以及肠道微生物群改变同时发生。硫酸软骨素（CS）是一种因其多种生物活性而闻名的多功能食物。作为一种硫酸盐益生元，CS在各种疾病中已显示出治疗潜力。在此，我们研究了孕期补充母源性CS对腺嘌呤饮食诱导的后代高血压的保护作用。在整个妊娠和哺乳期，给母鼠喂食常规饲料、0.5%腺嘌呤、3% CS或它们的组合。孕期补充母源性CS有效保护后代免受腺嘌呤饮食诱导的高血压影响。CS的这些有益作用与参与HS生成的酶3-巯基丙酮酸硫转移酶的肾mRNA和蛋白水平增加有关。此外，母源性CS处理显著增强了成年后代肠道微生物群的α多样性并改变了β多样性。具体而言，围产期CS处理促进了有益微生物如 和 的丰度。总之，围产期CS处理减轻了与母源性腺嘌呤饮食相关的后代高血压，表明早期给予硫酸盐益生元可能具有预防潜力。这些发现值得进一步开展转化研究以探索其临床意义。
