评估RIM系统和淋菌基因检测用于从临床标本中鉴定淋病奈瑟菌的效果。
Evaluation of the RIM system and Gono Gen test for identification of Neisseria gonorrhoeae from clinical specimens.
作者信息
Robinson A, Griffith S B, Moore D G, Carlson J R
出版信息
Diagn Microbiol Infect Dis. 1985 Mar;3(2):125-30. doi: 10.1016/0732-8893(85)90021-5.
RIM-N (Austin Biological Laboratories) and Gono Gen (Micro Media Systems) were evaluated for accuracy and compared with conventional carbohydrate degradation tests for identification of Neisseria gonorrhoeae. A total of 127 fresh clinical isolates of N. gonorrhoeae were tested; 118 (93%) were identified by RIM-N, and 100 (79%) yielded positive reactions with Gono Gen. Seventy nongonococcal isolates including other Neisseria species, Branhamella catarrhalis, Kingella kingae, and Moraxella species were evaluated to determine the specificity of the test methods. Both rapid tests were 100% specific in the identification of N. gonorrhoeae isolates. RIM-N was the most sensitive test for the identification of N. gonorrhoeae and offers a useful, more rapid alternative to conventional carbohydrate degradation methods.
对RIM-N（奥斯汀生物实验室）和Gono Gen（微媒体系统公司）进行了准确性评估，并与用于鉴定淋病奈瑟菌的传统碳水化合物降解试验进行了比较。共检测了127株淋病奈瑟菌的新鲜临床分离株；RIM-N鉴定出118株（93%），Gono Gen检测出100株（79%）呈阳性反应。对包括其他奈瑟菌属、卡他布兰汉菌、金氏金氏菌和莫拉菌属在内的70株非淋球菌分离株进行了评估，以确定检测方法的特异性。两种快速检测方法在鉴定淋病奈瑟菌分离株时特异性均为100%。RIM-N是鉴定淋病奈瑟菌最敏感的检测方法，是传统碳水化合物降解方法的一种有用且更快速的替代方法。
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