House L Caroline, Hasan Amer, Asnayanti Andi, Alrubaye Adnan A K, Pummill Jeff, Rhoads Douglas
John Brown University, Siloam Springs, AR 72761, USA.
Cell and Molecular Biology Pogram, University of Arkansas, Fayetteville, AR 72703, USA.
Microorganisms. 2024 Jul 29;12(8):1549. doi: 10.3390/microorganisms12081549.
We sequenced and assembled genomes for 17 isolates of isolated from osteomyelitis lesions in young broilers from two separate experiments where we induced lameness using a hybrid wire-litter flooring system. Whole genome comparisons using three different methods support a close relationship of genomes from both and . The data support three different lineages, which we designated as Lineage 1, Lineage 2, and Lineage 3, uniting these two species within an evolving complex. We present evidence for horizontal transfer between lineages of genomic regions from 50-440 kbp. The transfer of a 186 kbp region from Lineage 1 to Lineage 2 appears to have generated Lineage 3. Human-associated isolates appear to be limited to Lineages 2 and 3 but Lineage 2 appears to contain a higher number of human pathogenic isolates. The chicken isolates from our lameness trials included genomically diverse isolates from both Lineage 1 and 2, and isolates from both lineages were obtained from osteomyelitis lesions of individual birds. Our results expand the diversity of Staphylococci associated with osteomyelitis in poultry and suggest a high diversity in the microbiome of day-old chicks. Our data also support a reevaluation and unification of the taxonomic classifications of and .
我们对从两个独立实验中患有骨髓炎的年轻肉鸡病变部位分离出的17株菌株进行了基因组测序和组装，这两个实验中我们使用混合金属网垫料地板系统诱导跛行。使用三种不同方法进行的全基因组比较支持了来自两个物种的基因组之间的密切关系。数据支持三个不同的谱系，我们将其指定为谱系1、谱系2和谱系3，这两个物种在一个不断进化的复合体中合并在一起。我们提供了基因组区域在谱系之间水平转移的证据，转移范围为50 - 440 kbp。从谱系1到谱系2转移的一个186 kbp区域似乎产生了谱系3。与人类相关的分离株似乎仅限于谱系2和3，但谱系2似乎包含更多的人类致病分离株。我们跛行试验中的鸡分离株包括来自谱系1和2的基因组多样化分离株，并且两个谱系的分离株均从个体鸟类的骨髓炎病变中获得。我们的结果扩展了与家禽骨髓炎相关的葡萄球菌的多样性，并表明一日龄雏鸡的微生物组具有高度多样性。我们的数据还支持对两个物种的分类学分类进行重新评估和统一。
