Dos Santos Cintia Miranda, Baldivia Debora da Silva, de Castro David Tsuyoshi Hiramatsu, Carvalho José Tarciso de Giffoni, Oliveira Alex Santos, da Rocha Paola Dos Santos, Campos Jaqueline Ferreira, Balogun Sikiru Olaitan, de Oliveira Caio Fernando Ramalho, da Silva Denise Brentan, Carollo Carlos Alexandre, de Picoli Souza Kely, Dos Santos Edson Lucas
Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Universidade Federal da Grande Dourados, Dourados 79804-970, MS, Brazil.
Programa de Pós-Graduação em Ciências de Saúde, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados 79804-970, MS, Brazil.
Pharmaceuticals (Basel). 2024 Jul 23;17(8):974. doi: 10.3390/ph17080974.
is a species found in the Cerrado and used in folk medicine as a vermifuge and in the treatment of poisonous snakebites accidents. In this work, we identified the main secondary metabolites present in ethanolic extracts of the leaves (ELSR) and roots (ERSR) of and evaluated the potential cytoprotective effect against cellular macromolecular damage, as well as the cytotoxic properties of the extracts on the K562 and Jurkat leukemic cell lines. The identification of metabolites was carried out by liquid chromatography coupled with mass spectrometry. The antioxidant activities were investigated by direct ABTS and DPPH radical scavenging methods, protection against oxidative damage in proteins, and DNA. Cytotoxic properties were investigated against healthy cells, isolated from human peripheral blood (PBMC) and leukemic cell lines. The leaf extracts contained catechin, rutin, epigallocatechin derivatives, kaempferol glycosides, luteolin, and dimeric and trimeric procyanidins, while the root extract profile showed obtusichromoneside derivatives, 2-methoxystypandrone, stilbene derivatives, naphthopyranones, and flavanone derivatives. The extracts showed antioxidant activity, with an IC of 4.86 ± 0.51 μg/mL and 8.33 ± 0.90 μg/mL in the ABTS assay for ELSR and ERSR, respectively. Furthermore, in the DPPH assay, the IC was 19.98 ± 1.96 μg/mL for ELSR and 13.37 ± 1.05 μg/mL for ERSR. The extracts protected macromolecules against oxidative damage at concentrations of 5 μg/mL. The cytotoxicity test against leukemic strains was observed after 24 and 48 h of treatment. After 48 h, results against the K562 cell line demonstrate an IC of 242.54 ± 2.38 μg/mL and 223.00 ± 2.34 μg/mL for ELSR and ERSR, respectively. While against the Jurkat cell line, these extracts showed an IC of 171.45 ± 2.25 μg/mL and 189.30 ± 2.27 μg/mL, respectively. The results pertaining to PBMC viability demonstrated that the extracts showed selectivity for the leukemic cell lines. Together, our results reveal that the leaves and roots of have completely distinct and complex chemical compositions and expand their significant pharmacological potential in oxidative stress and leukemia conditions.
是一种发现于塞拉多地区的物种，在民间医学中用作驱虫剂和治疗毒蛇咬伤事故。在这项工作中，我们鉴定了该植物叶片乙醇提取物（ELSR）和根乙醇提取物（ERSR）中存在的主要次生代谢产物，并评估了其对细胞大分子损伤的潜在细胞保护作用，以及提取物对K562和Jurkat白血病细胞系的细胞毒性特性。代谢产物的鉴定通过液相色谱-质谱联用进行。通过直接ABTS和DPPH自由基清除方法、对蛋白质和DNA氧化损伤的保护作用研究抗氧化活性。针对从人外周血分离的健康细胞（PBMC）和白血病细胞系研究细胞毒性特性。叶片提取物含有儿茶素、芦丁、表没食子儿茶素衍生物、山奈酚糖苷、木犀草素以及二聚体和三聚体原花青素，而根提取物的成分显示有钝叶色酮苷衍生物、2-甲氧基斯替潘酮、芪衍生物、萘并吡喃酮和黄烷酮衍生物。提取物显示出抗氧化活性，在ABTS试验中，ELSR和ERSR的IC50分别为4.86±0.51μg/mL和8.33±0.90μg/mL。此外，在DPPH试验中，ELSR的IC50为19.98±1.96μg/mL，ERSR的IC50为13.37±1.05μg/mL。提取物在5μg/mL浓度下可保护大分子免受氧化损伤。在处理24小时和48小时后观察针对白血病菌株的细胞毒性试验。48小时后，针对K562细胞系的结果显示，ELSR和ERSR的IC50分别为242.54±2.38μg/mL和223.00±2.34μg/mL。而针对Jurkat细胞系，这些提取物的IC50分别为171.45±2.25μg/mL和189.30±2.27μg/mL。与PBMC活力相关的结果表明，提取物对白血病细胞系具有选择性。总之，我们的结果表明该植物的叶和根具有完全不同且复杂的化学成分，并拓展了它们在氧化应激和白血病病症中的重要药理潜力。
