Institute of insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
State Key Lab of Rice Biology, Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects, and Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, 310058, China.
Sci Data. 2024 Aug 29;11(1):944. doi: 10.1038/s41597-024-03779-y.
Diomorus aiolomorphi Kamijo (Hymenoptera: Torymidae) is an inquiline of gall maker Aiolomorphus rhopaloides Walker (Hymenoptera: Eurytomidae). They are of significant economic significance and predominantly inhabit bamboo forest. So far, only four scaffold-level genomes have been published for the family Torymidae. In this study, we present a high-quality genome assembly of D. aiolomorphi at the chromosome level, achieved through the integration of Nanopore (ONT) long-read, Illumina pair-end DNA short-read, and High-through Chromosome Conformation Capture (Hi-C) sequencing methods. The final assembly was 1,084.56 Mb in genome size, with 1,083.41 Mb (99.89%) assigned to five pseudochromosomes. The scaffold N50 length reached 224.87 Mb, and the complete Benchmarking Universal Single-Copy Orthologs (BUSCO) score was 97.3%. The genome contained 762.12 Mb of repetitive elements, accounting for 70.27% of the total genome size. A total of 18,011 protein-coding genes were predicted, with 17,829 genes being functionally annotated. The high-quality genome assembly of D. aiolomorphi presented in this study will serve as a valuable genomic resource for future research on parasitoid wasps. The results of this study may also contribute to the development of biological control strategies for pest management in bamboo forests, enhancing ecological balance and economic sustainability.
姬小蜂科的双带盘绒茧蜂（Hymenoptera： Torymidae）是钻蛀性瘿蜂 Aiolomorphus rhopaloides Walker（Hymenoptera： Eurytomidae）的一种内寄生蜂。它们具有重要的经济意义，主要栖息在竹林中。迄今为止，仅发表了该科的四个支架级基因组。在这项研究中，我们通过整合纳米孔（ONT）长读长、Illumina 双端 DNA 短读长和高通量染色体构象捕获（Hi-C）测序方法，提供了双带盘绒茧蜂染色体水平的高质量基因组组装。最终组装的基因组大小为 1,084.56 Mb，其中 1,083.41 Mb（99.89%）分配到五个假染色体上。支架 N50 长度达到 224.87 Mb，完整的基准通用单拷贝直系同源物（BUSCO）评分为 97.3%。基因组包含 762.12 Mb 的重复元件，占总基因组大小的 70.27%。共预测到 18,011 个蛋白质编码基因，其中 17,829 个基因具有功能注释。本研究中提供的双带盘绒茧蜂高质量基因组组装将成为未来寄生蜂研究的宝贵基因组资源。本研究的结果也可能有助于竹林害虫生物防治策略的制定，增强生态平衡和经济可持续性。
