Zhen Li, Huang Yi, Bi Xuewen, Gao Anyu, Peng Linlin, Chen Yong
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China.
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural Affairs, Daqing, China.
Front Microbiol. 2024 Aug 19;15:1422272. doi: 10.3389/fmicb.2024.1422272. eCollection 2024.
To study the effect of melatonin supplementation on the gut microbes of broilers, 160 healthy 3-week-old Ross 308 broilers with similar body weights were selected and randomly divided into four groups (M0, M20, M40, and M80) supplemented with 0, 20, 40, or 80 mg/kg melatonin. The results showed that the abundance-based coverage estimator (ACE) index of cecum microorganisms was significantly lower in the M80 group. The dominant phyla of intestinal contents in the M0, M20, M40, and M80 groups were Bacteroidetes and Firmicutes. The M40 group showed an increase in the relative abundance of Bacteroidetes spp. in the intestine, while the relative abundance of Ruminococcus spp. in the intestine of the M20, M40, and M80 groups was significantly greater than that of the M0 group. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses revealed that the supplementation of melatonin increases the expression of genes related to cellular processes (cell motility, cell growth and death, and cellular community-eukaryotes), environmental information processing (membrane transport and signal transduction), and genetic information processing (transport and transcription), and Cluster of Orthologous Groups (COG) of proteins functional analyses revealed that the supplementation of melatonin resulted in a significant increase in cellular processes and signaling (cell motility, signal transduction mechanisms, intracellular trafficking, secretion, and vesicular transport), information storage and processing (RNA processing and modification, chromatin structure and dynamics, translation, ribosomal structure, and biogenesis), metabolism (energy production and conversion, lipid transportation and metabolism, inorganic ion transport and metabolism, secondary metabolite biosynthesis, transport, and catabolism), and poorly characterized (general function prediction only). In summary, supplementation of feed with melatonin can increase the diversity of intestinal microorganisms and the relative abundance of Bacteroides and Firmicutes in the cecum, improve digestive ability and nutrient absorption ability, and positively regulate the metabolic ability of broilers.
为研究补充褪黑素对肉鸡肠道微生物的影响，选取160只体重相近的3周龄健康罗斯308肉鸡，随机分为四组（M0、M20、M40和M80），分别补充0、20、40或80mg/kg褪黑素。结果显示，M80组盲肠微生物的基于丰度的覆盖率估计器（ACE）指数显著降低。M0、M20、M40和M80组肠道内容物的优势菌门为拟杆菌门和厚壁菌门。M40组肠道中拟杆菌属的相对丰度增加，而M20、M40和M80组肠道中瘤胃球菌属的相对丰度显著高于M0组。京都基因与基因组百科全书（KEGG）功能分析表明，补充褪黑素可增加与细胞过程（细胞运动、细胞生长和死亡以及细胞群落-真核生物）、环境信息处理（膜运输和信号转导）以及遗传信息处理（运输和转录）相关基因的表达，直系同源蛋白簇（COG）功能分析表明，补充褪黑素导致细胞过程和信号传导（细胞运动、信号转导机制、细胞内运输、分泌和囊泡运输）、信息存储和处理（RNA加工和修饰、染色质结构和动力学、翻译、核糖体结构和生物发生）、代谢（能量产生和转换、脂质运输和代谢、无机离子运输和代谢、次级代谢产物生物合成、运输和分解代谢）以及功能特征不明确（仅一般功能预测）方面显著增加。综上所述，在饲料中添加褪黑素可增加肠道微生物多样性以及盲肠中拟杆菌和厚壁菌的相对丰度，提高消化能力和营养吸收能力，并对肉鸡的代谢能力产生积极调节作用。
