Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, 102206, China.
Center for Biosafety Mega-Science, Chinese Academy of Science, Wuhan, 430071, Hubei, China.
Arch Virol. 2024 Sep 3;169(9):192. doi: 10.1007/s00705-024-06117-0.
Nanoparticles have gained attention as potential antiviral agents, but the effects of graphene oxide nanoparticles (GONPs) on influenza virus remain unclear. In this study, we evaluated the antiviral activity of GONPs against influenza virus strain A/Hunan-Lengshuitan/11197/2013(H9N2). Our results show that GONPs with a diameter of 4 nm exerted an antiviral effect, whereas those with a diameter of 400 nm had no effect. Treatment with 4-nm GONPs reduced viral titers by more than 99% and inhibited viral nucleoprotein expression in a dose-dependent manner. We also confirmed that 4-nm GONPs inhibited the infectivity of H9N2 in MDCK cells. A transmission electron microscopic analysis revealed morphological abnormalities in the GONP-treated virus, including the destruction of the envelope glycoprotein spikes and an irregular shape, suggesting that GONPs cause the destruction of the viral coat proteins. Our results highlight the potential utility of GONPs in the prevention and treatment of viral infections, especially those of emerging and re-emerging viruses.
纳米颗粒作为潜在的抗病毒药物引起了关注，但氧化石墨烯纳米颗粒（GONP）对流感病毒的影响尚不清楚。在本研究中，我们评估了 GONP 对流感病毒株 A/Hunan-Lengshuitan/11197/2013（H9N2）的抗病毒活性。我们的结果表明，直径为 4nm 的 GONP 发挥了抗病毒作用，而直径为 400nm 的 GONP 则没有作用。用 4nm GONP 处理可使病毒滴度降低 99%以上，并呈剂量依赖性抑制病毒核蛋白表达。我们还证实，4nm GONP 抑制了 H9N2 在 MDCK 细胞中的感染性。透射电子显微镜分析显示，GONP 处理的病毒存在形态异常，包括包膜糖蛋白刺突的破坏和不规则形状，表明 GONP 导致病毒外壳蛋白的破坏。我们的结果强调了 GONP 在预防和治疗病毒感染，特别是新发和再发病毒感染方面的潜在应用价值。
