Faculty of Veterinary Medicine, Federal University of Uberlândia, Av. Pará, 1720, Campus Umuarama, Bloco 2 E, CEP 38405-320, Uberlândia, Minas Gerais, Brazil.
Faculty of Veterinary Medicine, Federal University of Bahia, Av. Milton Santos, 500, Ondina, CEP 40170-110, Salvador, Bahia, Brazil.
Curr Microbiol. 2024 Sep 3;81(10):339. doi: 10.1007/s00284-024-03872-w.
Bacterial spores in materials and equipment pose significant biosecurity risks, making effective disinfection crucial. This study evaluated Ortho-phthalaldehyde (OPA) and a quaternary ammonia-glutaraldehyde solution (AG) for inactivating spores of Bacillus thuringiensis (BT), B. cereus (BC), and two strains of B. velezensis (BV1 and BV2). Spores of BV1 and BT were treated with 22.5 mg/m OPA by dry fumigation or 1 mg/mL AG by spray for 20 min, according to the manufacturer's recommendation. As no sporicidal effect was observed, OPA was tested at 112.5 mg/m for 40 min, showing effectiveness for BT but not for BV1. Minimum bactericidal concentration (MBC) tests revealed higher MBC values for glutaraldehyde, prompting an overnight test with 112.5 mg/m OPA by dry fumigation and 50 mg/mL AG by spray, using formaldehyde as a control. AG reduced all Bacillus strains, but with limited sporicidal effect. OPA was sporicidal for BT and BV1 but not for BC and BV2, indicating a strain-dependent effect. Formaldehyde performed better overall but did not completely inactivate BV2 spores. Our findings suggest that OPA and AG have potential as formaldehyde replacements in wet disinfection procedures.
材料和设备中的细菌孢子构成了重大的生物安全风险，因此有效消毒至关重要。本研究评估了邻苯二醛（OPA）和季铵盐-戊二醛溶液（AG）对苏云金芽孢杆菌（BT）、蜡状芽孢杆菌（BC）和两种韦氏芽孢杆菌（BV1 和 BV2）孢子的灭活效果。根据制造商的建议，采用 22.5mg/m 的 OPA 进行干法熏蒸或 1mg/mL 的 AG 进行喷雾，处理 BV1 和 BT 的孢子 20 分钟。由于没有观察到杀孢子效果，OPA 的测试浓度增加到 112.5mg/m 并作用 40 分钟，结果显示对 BT 有效，但对 BV1 无效。最低杀菌浓度（MBC）测试显示戊二醛的 MBC 值更高，因此进行了一项 overnight 测试，采用 112.5mg/m 的 OPA 进行干法熏蒸和 50mg/mL 的 AG 进行喷雾，甲醛作为对照。AG 可降低所有芽孢杆菌菌株，但杀孢子效果有限。OPA 对 BT 和 BV1 具有杀孢子作用，但对 BC 和 BV2 无效，表明存在菌株依赖性。甲醛的总体效果更好，但不能完全使 BV2 孢子失活。我们的研究结果表明，OPA 和 AG 有可能替代甲醛用于湿式消毒程序。
