Meat Science and Animal Biologics Discovery, Department of Animal & Dairy Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, USA.
Methods Mol Biol. 2025;2852:273-288. doi: 10.1007/978-1-0716-4100-2_18.
The standardization of the microbiome sequencing of poultry rinsates is essential for generating comparable microbial composition data among poultry processing facilities if this technology is to be adopted by the industry. Samples must first be acquired, DNA must be extracted, and libraries must be constructed. In order to proceed to library sequencing, the samples should meet quality control standards. Finally, data must be analyzed using computer bioinformatics pipelines. This data can subsequently be incorporated into more advanced computer algorithms for risk assessment. Ultimately, *a uniform sequencing pipeline will enable both the government regulatory agencies and the poultry industry to identify potential weaknesses in food safety.This chapter presents the different steps for monitoring the population dynamics of the microbiome in poultry processing using 16S rDNA sequencing.
如果该技术要被家禽加工业采用，那么对家禽冲洗物的微生物组测序进行标准化是生成可比较的微生物组成数据的关键。首先必须采集样本，提取 DNA，构建文库。为了进行文库测序，样本应符合质量控制标准。最后，必须使用计算机生物信息学管道对数据进行分析。该数据随后可被纳入更先进的计算机算法中，以进行风险评估。最终，统一的测序管道将使政府监管机构和家禽行业能够识别食品安全方面的潜在弱点。本章介绍了使用 16S rDNA 测序监测家禽加工过程中微生物组种群动态的不同步骤。
