Silva Jacqueline de Azerêdo, Santos George Gonçalves Dos, Ribeiro Iorrana Índira Dos Anjos, Silva Ana Maria Guerreiro Braga da, Barreto Isabela Cerqueira, Matos Marcos Almeida, Barreto Maurício Andrade, Miguel Fúlvio Borges
Centro de Medicina Hiperbárica do Nordeste (CMHN), Salvador, BA, Brasil.
Centro de Ciências da Saúde (CCS), Universidade Federal do Recôncavo da Bahia (UFRB), Santo Antônio de Jesus, BA, Brasil.
Rev Bras Ortop (Sao Paulo). 2024 Sep 4;59(4):e519-e525. doi: 10.1055/s-0044-1787768. eCollection 2024 Aug.
The present study aims to analyze histomorphometrically the repair of a non-critical bone defect after implantation of hydroxyapatite (HA) microspheres substituted by magnesium (Mg). Thirty rats were distributed into 3 experimental groups, evaluated at 15 and 45 days postoperatively: HAG (bone defect filled with HA microspheres); HAMgG (bone defect filled with HA microspheres replaced with 1 mol% Mg), and CG (bone defect without implantation of biomaterials). After 15 days, the biomaterials filled the entire defect extent, forming a new osteoid matrix between the microspheres. In the CG, this neoformation was restricted to the edges with the deposition of loose connective tissue with reduced thickness. At 45 days, new bone formation filled almost the entire extension of the bone defect in the 3 groups, with statistically significant osteoid deposition in the CG despite the reduced thickness compared with the HAG and HAMgG. The groups with biomaterial implantation displayed a more abundant osteoid matrix than at 15 days. The biomaterials studied showed biocompatibility, osteoconductivity, and bioactivity. The Mg concentration in the substituted HA did not stimulate more significant bone formation than HA without this ion.
本研究旨在通过组织形态计量学分析镁（Mg）取代的羟基磷灰石（HA）微球植入后非关键性骨缺损的修复情况。30只大鼠被分为3个实验组，在术后15天和45天进行评估：HAG组（骨缺损填充HA微球）；HAMgG组（骨缺损填充1 mol% Mg取代的HA微球），以及CG组（骨缺损未植入生物材料）。术后15天，生物材料填充了整个缺损范围，在微球之间形成了新的类骨质基质。在CG组中，这种新形成仅限于边缘，伴有厚度减小的疏松结缔组织沉积。术后45天，3组中几乎整个骨缺损范围均有新骨形成，尽管与HAG组和HAMgG组相比CG组的厚度减小，但类骨质沉积具有统计学意义。植入生物材料的组显示出比术后15天更丰富的类骨质基质。所研究的生物材料具有生物相容性、骨传导性和生物活性。取代HA中的Mg浓度并未比不含该离子的HA刺激更显著的骨形成。
