血凝抑制试验与酶联免疫吸附测定法在风疹病毒抗体检测中的比较
Comparison of hemagglutination inhibition test and enzyme-linked immunosorbent assay for determining antibody to rubella virus.
作者信息
Shekarchi I C, Sever J L, Tzan N, Ley A, Ward L C, Madden D
出版信息
J Clin Microbiol. 1981 May;13(5):850-4. doi: 10.1128/jcm.13.5.850-854.1981.
Abstract
The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum samples from the Collaborative Perinatal Project, NIH/NINCDS. The tests were found to be comparable in their ability to detect positive and negative sera, rises in titers, and seroconversions. When a purified antigen and carefully prepared reagents were used, ELISA was found to be as accurate and reliable as HAI. ELISA required no pretreatment of serum, could easily be automated, and was less time-consuming than HAI.
摘要
通过检测25组感染前后的配对血清、10组疾病急性期和恢复期采集的血清，并对来自美国国立卫生研究院/国立神经疾病与中风研究所围产期协作项目的700份血清样本进行筛查，比较了用于检测风疹病毒抗体的血凝抑制试验（HAI）和酶联免疫吸附测定（ELISA）。结果发现，这两种检测方法在检测阳性和阴性血清、滴度升高以及血清转化方面的能力相当。当使用纯化抗原和精心制备的试剂时，ELISA与HAI一样准确可靠。ELISA无需对血清进行预处理，易于自动化，且比HAI耗时少。
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