Alsalamah Sulaiman A, Alghonaim Mohammed Ibrahim, Jusstaniah Mohammed, Abdelghany Tarek M
Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia.
University Medical Service Center, Building 70, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Life (Basel). 2023 Aug 30;13(9):1839. doi: 10.3390/life13091839.
, known as henna, has traditionally been utilized in cosmetics and folk medicine because of their valuable health effects. A lack of information about the processes that increase or decrease release, as well as the biological activities of constituents of natural origin, is an important pharmacological problem. This investigation evaluates the influence of moist heat on the flavonoid and phenolic contents of henna powder and their biological activities. HPLC analysis reflected the existence of 20 and 19 compounds of flavonoids and phenolics in the extract of unpre-treated henna by moist heat (UPMH) and pre-treated henna by moist heat (PMH). Several compounds such as chlorogenic acid, ellagic acid, rutin, rosmarinic acid, kaempferol, and pyrocatechol occurred with high concentrations of 57,017.33, 25,821.09, 15,059.88, 6345.08, 1248.42, and 819.19 µg/mL UPMH while occurred with low concentrations of 44,286.51, 17,914.26, 3809.85, 5760.05, 49.01, and 0.0 µg/mL, respectively in PMH. , , and were more affected by UPMH with inhibition zones of 30.17 ± 0.29, 27 ± 0.5, and 29 ± 1.5 mm than PMH with inhibition zones of 29 ± 0.5, 25.33 ± 0.58, and 24.17 ± 0.29 mm, respectively. UPMH henna exhibited less MIC and MFC against the tested yeasts than PMH. Moreover, UPMH henna showed good wound healing, where the rat of migration, wound closure %, and area difference % were 14.806 um, 74.938 um, and 710.667% compared with PMH henna 11.360 um, 59.083 um, 545.333%, respectively. Antioxidant activity of UPMH and PMH henna. Promising antioxidant activity was recorded for both UPMH or PMH henna with IC 5.46 µg/mL and 7.46 µg/mL, respectively. The docking interaction of chlorogenic acid and ellagic acid with the crystal structures of (4ZZT) and (4YDE) was examined. The biological screening demonstrated that the compounds had favorable docking results with particular proteins. Chlorogenic acid had robust behavior in the (4ZZT) active pocket and displayed a docking score of -7.84379 Kcal/mol, higher than ellagic acid's -6.18615 Kcal/mol.
[植物名称]，又称指甲花，由于其具有宝贵的健康功效，传统上一直被用于化妆品和民间医学。缺乏关于增加或减少释放过程以及天然成分生物活性的信息是一个重要的药理学问题。本研究评估了湿热对指甲花粉中黄酮类和酚类成分及其生物活性的影响。高效液相色谱分析表明，未经湿热处理的指甲花提取物（UPMH）和经湿热处理的指甲花提取物（PMH）中分别存在20种和19种黄酮类和酚类化合物。几种化合物，如绿原酸、鞣花酸、芦丁、迷迭香酸、山奈酚和邻苯二酚，在UPMH中的浓度较高，分别为57017.33、25821.09、15059.88、6345.08、1248.42和819.19μg/mL，而在PMH中的浓度较低，分别为44286.51、17914.26、3809.85、5760.05、49.01和0.0μg/mL。[具体菌株1]、[具体菌株2]和[具体菌株3]受UPMH的影响更大，抑菌圈分别为30.17±0.29、27±0.5和29±1.5mm，而受PMH的影响较小，抑菌圈分别为29±0.5、25.33±0.58和24.17±0.29mm。UPMH指甲花对受试酵母的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）低于PMH。此外，UPMH指甲花显示出良好的伤口愈合效果，迁移率、伤口闭合率和面积差异率分别为14.806μm、74.938μm和710.667%，而PMH指甲花分别为为11.360μm、59.083μm、545.333%。UPMH和PMH指甲花的抗氧化活性。UPMH和PMH指甲花都具有良好的抗氧化活性，IC50分别为5.46μg/mL和7.46μg/mL。研究了绿原酸和鞣花酸与[具体蛋白1]（4ZZT）和[具体蛋白2]（4YDE）晶体结构的对接相互作用。生物筛选表明，这些化合物与特定蛋白质具有良好的对接结果。绿原酸在[具体蛋白1]（4ZZT）活性口袋中表现出较强的活性，对接分数为-7.84379千卡/摩尔，高于鞣花酸的-6.18615千卡/摩尔。
