Fich A, Arber N, Sestieri M, Zajicek G, Rachmilewitz D
Gastroenterology. 1985 Jul;89(1):57-61. doi: 10.1016/0016-5085(85)90745-0.
The effect of misoprostol and cimetidine on gastric cell turnover was studied. Endoscopic biopsy specimens of fundic and antral mucosa were obtained from duodenal ulcer patients before and after 4 wk of therapy with cimetidine 1.2 g/day or misoprostol 800 micrograms/day. Biopsy specimens were incubated with [3H]thymidine. Glandular column length and number of labeled cells were determined after autoradiography. There was no significant difference in column length of antral or fundic glands before or after therapy with cimetidine and misoprostol. The number of antral and fundic labeled cells was significantly decreased after misoprostol treatment (3.6 +/- 0.3 and 4.6 +/- 0.4, mean +/- SE), as opposed to their respective number before therapy (6.9 +/- 0.5 and 8.3 +/- 0.8) (p less than 0.01). On the other hand, after treatment with cimetidine, the number of antral and fundic labeled cells was significantly higher (11.8 +/- 0.9 and 7.5 +/- 1.0, respectively) as compared with their number before therapy (5.7 +/- 0.5 and 5.6 +/- 0.6, respectively). The decreased gastric cell turnover induced by misoprostol indicates that the trophic effect of prostanoids on gastric mucosa is not due to an increase in cellular kinetics. The increased gastric cell turnover induced by cimetidine may contribute to its therapeutic effect in peptic ulcer disease.
研究了米索前列醇和西咪替丁对胃细胞更新的影响。从十二指肠溃疡患者中获取胃底和胃窦黏膜的内镜活检标本，分别在接受每日1.2克西咪替丁或每日800微克米索前列醇治疗4周前后进行取材。活检标本用[³H]胸腺嘧啶核苷进行孵育。放射自显影后测定腺管柱状长度和标记细胞数量。西咪替丁和米索前列醇治疗前后，胃窦或胃底腺管的柱状长度无显著差异。米索前列醇治疗后，胃窦和胃底标记细胞数量显著减少（分别为3.6±0.3和4.6±0.4，均值±标准误），与治疗前各自的数量（分别为6.9±0.5和8.3±0.8）相比（p＜0.01）。另一方面，西咪替丁治疗后，胃窦和胃底标记细胞数量显著高于治疗前（分别为11.8±0.9和7.5±1.0）（治疗前分别为5.7±0.5和5.6±0.6）。米索前列醇诱导的胃细胞更新减少表明，前列腺素对胃黏膜的营养作用并非由于细胞动力学增加所致。西咪替丁诱导的胃细胞更新增加可能有助于其在消化性溃疡疾病中的治疗作用。
