Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Department of Microbiology, Research Institute of Medical Entomology, General Organization for Teaching Hospitals and Institutes, Giza, Egypt.
Curr Microbiol. 2024 Sep 11;81(11):352. doi: 10.1007/s00284-024-03864-w.
This study aimed to screen the bioactive components in Streptococcus equinus WC (SE-WC) and Limosilactobacillus reuteri GM (LR-GM) and estimate the therapeutic role in Ehrlich solid tumors (EST) mice model. Forty-four male albino EST mice were assigned into 7 groups and treated daily for 2 weeks, including the EST group, the EST mice that received SE-WC at a low or a high dose (0.5 ml *10 or 0.5 ml *10 cfu), the EST mice that received LR-GM at the low or the high dose (0.5 ml *10 or 0.5 ml *10 cfu), and the EST mice that received SE-WC plus LR-GM at the low or the high dose. Tumors were harvested, weighed, examined, and used for the determination of apoptosis-related gene expression. Samples of the intestine, liver, and kidney were gathered for histological examination. The GC-MS identified 24 and 36 bioactive compounds in SE-WC and LR-GM, respectively. The main compound in SE-WC was lupeol; however, the main compound in LR-GM was retinaldehyde. EST mice showed disturbances in Bcl-2, Bax, and p53 mRNA expression along with histological changes in the intestine, liver, and kidney. Administration of both bacterial strains reduced the tumor weight, alleviated the disturbances in the gene expression, and improved the histological structure of the intestine, liver, and kidney in a dose-dependent. Moreover, LR-GM was more effective than SE-WC due to its higher content of bioactive compounds. It could be concluded that these strains of probiotics are promising for the treatment of solid tumors.
本研究旨在筛选马链球菌兽疫亚种（SE-WC）和鼠李糖乳杆菌 GM（LR-GM）中的生物活性成分，并评估其在艾氏腹水瘤（EST）小鼠模型中的治疗作用。将 44 只雄性白化 EST 小鼠随机分为 7 组，每天接受治疗 2 周，包括 EST 组、SE-WC 低、高剂量（0.5ml10 或 0.5ml10cfu）组、LR-GM 低、高剂量（0.5ml10 或 0.5ml10cfu）组、SE-WC+LR-GM 低、高剂量组。采集肿瘤，称重，检查，并用于测定凋亡相关基因表达。采集肠、肝、肾组织样本进行组织学检查。GC-MS 分别鉴定出 SE-WC 和 LR-GM 中的 24 种和 36 种生物活性化合物。SE-WC 的主要化合物为羽扇豆醇；然而，LR-GM 的主要化合物为视黄醛。EST 小鼠表现出 Bcl-2、Bax 和 p53 mRNA 表达紊乱，以及肠、肝、肾组织学改变。两种细菌菌株的给药均降低了肿瘤重量，减轻了基因表达紊乱，并改善了肠、肝、肾的组织学结构，呈剂量依赖性。此外，由于 LR-GM 中生物活性化合物含量较高，因此比 SE-WC 更有效。可以得出结论，这些益生菌菌株有望用于治疗实体瘤。
