Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Disease Control and Prevention, Dali, Yunnan, China.
The Centre for Infection and Immunity Study, School of Medicine, Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong, China.
PLoS Negl Trop Dis. 2024 Sep 12;18(9):e0012478. doi: 10.1371/journal.pntd.0012478. eCollection 2024 Sep.
Seoul orthohantavirus (SEOV) has been identified as one of the main causative agents of hemorrhagic fever with renal syndrome (HFRS) in China. The virus was found circulating in rodent populations in almost all provinces of the country, reflecting the wide distribution of HFRS. Here, using the direct immunofluorescence assay (DFA) and real-time quantitative reverse transcription PCR (qRT-PCR) approach, we performed screening in 1784 small mammals belonging to 14 species of three orders captured in the main areas of HFRS endemicity in Yunnan province (southwestern China) and identified 37 SEOV-positive rats (36 Rattus norvegicus and 1 Rattus tanezumi). A 3-year surveillance of HFRS epidemics and dynamics of rodent reservoir density and virus prevalence implied a potential correlation between them. The subsequent meta-transcriptomic sequencing and phylogenetic analyses revealed three SEOV variants, among which two are completely novel. The ancestral character state reconstruction (ACSR) analysis based on both novel variants and documented strains from 5 continents demonstrated that SEOV appeared to originate near the southwestern area (Yunnan-Kweichow Plateau) of China, then could spread to other regions and countries by their rodent carriers, resulting in a global distribution today. In summary, these data furthered the understanding regards genetic diversity and the potential origin for SEOV. However, the expanding endemic foci in the province suggest that the virus is spreading over a wider region and is much more diverse than previous depicted, which means that increased sampling is necessary.
首尔病毒（SEOV）已被确定为中国肾综合征出血热（HFRS）的主要病原体之一。该病毒在全国几乎所有省份的啮齿动物种群中传播，反映了 HFRS 的广泛分布。在这里，我们使用直接免疫荧光检测（DFA）和实时定量逆转录 PCR（qRT-PCR）方法，对从中国西南部云南省 HFRS 流行的主要地区捕获的 14 种三个目共 1784 只小型哺乳动物进行了筛选，鉴定出 37 只 SEOV 阳性大鼠（36 只挪威鼠和 1 只褐家鼠）。对 HFRS 流行情况和啮齿动物储存密度及病毒流行率的 3 年监测表明，两者之间存在潜在的相关性。随后的元转录组测序和系统发育分析揭示了三种 SEOV 变体，其中两种是完全新型的。基于新型变体和来自五大洲的记录菌株的祖先特征状态重建（ACSR）分析表明，SEOV 似乎起源于中国西南部地区（云南-贵州高原），然后可以通过其啮齿动物载体传播到其他地区和国家，从而导致其在全球范围内分布。总之，这些数据进一步了解了 SEOV 的遗传多样性和潜在起源。然而，该省不断扩大的流行范围表明，该病毒正在向更广泛的地区传播，并且比以前描述的更加多样化，这意味着需要增加采样。
