Mitchell H K, Petersen N S, Buzin C H
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4969-73. doi: 10.1073/pnas.82.15.4969.
The 70-kDa heat shock protein of Drosophila decays in vivo at a much faster rate than other abundantly labeled proteins. Degradation also occurs in vitro, even during electrophoresis. It appears that this degradation is not mediated by a general protease and that the 70-kDa heat shock protein has a slow proteolytic action upon itself. Heat-induced proteins in CHO cells and a mouse cell line also degrade spontaneously in vitro, as do certain non-heat shock proteins from Drosophila tissues as well as the cell lines.
果蝇的70 kDa热休克蛋白在体内的降解速度比其他大量标记的蛋白质快得多。体外也会发生降解，甚至在电泳过程中也是如此。这种降解似乎不是由一般的蛋白酶介导的，而且70 kDa热休克蛋白自身具有缓慢的蛋白水解作用。CHO细胞和小鼠细胞系中的热诱导蛋白在体外也会自发降解，果蝇组织以及细胞系中的某些非热休克蛋白也是如此。
