Selective microextraction of erythrosine (E127) in foodstuffs using a new generation high-density type-V deep eutectic solvent.

A novel and selective (deep eutectic solvent) DES-based microextraction method was established for the first time, utilizing a synthesized new generation High-Density Type-V DES for monitoring the dye Erythrosine (E127) in various foodstuffs and drugs. Type-V DES was created from acetophenone and diphenylamine at 3:1 M ratio. The pH, DES amount, and vortex time were optimized using Box-Behnken Design (BBD) of Response Surface Methodology (RSM). The quadratic microextraction model with R = 0.9982 was obtained. The limit of detection, preconcentration factor and linear dynamic range were determined to be 12 μg/L, 50 and 41-4000 μg/L, respectively. Effects of matrix components were examined. The developed High-Density Type-V Deep Eutectic Solvent Microextraction (HD-V-DES-ME) method was applied to foodstuffs and drugs to monitor their E127 contents and subsequently validated by applying spiked tests to real samples, with recoveries ranging between 94 and 101 %. The indexes of environmental friendliness and practicality for the method were evaluated using the Analytical GREEnness metric approach tool (AGREE) and the Blue Applicability Grade Index tool (BAGI), respectively.