Zhang Xiaoxue, Yi Zongyi, Tang Wei, Wei Wensheng
Changping Laboratory, Beijing 102206, China.
Biomedical Pioneering Innovation Center, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.
Biophys Rep. 2024 Aug 31;10(4):191-200. doi: 10.52601/bpr.2024.240010.
Mitochondrial base editing tools hold great promise for the investigation and treatment of mitochondrial diseases. Mitochondrial DNA base editors (mitoBEs) integrate a programmable transcription-activator-like effector (TALE) protein with single-stranded DNA deaminase (TadA8e-V106W, APOBEC1, .) and nickase (MutH, Nt.BspD6I(C), .) to achieve heightened precision and efficiency in mitochondrial base editing. This innovative mitochondrial base editing tool exhibits a number of advantages, including strand-selectivity for editing, high efficiency, and the capacity to perform diverse types of base editing on the mitochondrial genome by employing various deaminases. In this context, we provide a detailed experimental protocol for mitoBEs to assist others in achieving proficient mitochondrial base editing.
线粒体碱基编辑工具在研究和治疗线粒体疾病方面具有巨大潜力。线粒体DNA碱基编辑器（mitoBEs）将可编程的转录激活样效应因子（TALE）蛋白与单链DNA脱氨酶（TadA8e-V106W、APOBEC1等）和切口酶（MutH、Nt.BspD6I(C)等）整合，以提高线粒体碱基编辑的精度和效率。这种创新的线粒体碱基编辑工具具有许多优点，包括编辑的链选择性、高效率以及通过使用各种脱氨酶对线粒体基因组进行多种类型碱基编辑的能力。在此背景下，我们提供了一份针对mitoBEs的详细实验方案，以帮助其他人实现熟练的线粒体碱基编辑。
