Department of Research, Cancer Registry of Norway, Norwegian Institute of Public Health, Oslo, Norway.
Department of Tumor Biology, Oslo University Hospital, Oslo, Norway.
mSystems. 2024 Oct 22;9(10):e0073424. doi: 10.1128/msystems.00734-24. Epub 2024 Sep 17.
We have previously demonstrated an association between increased abundance of and colorectal cancer (CRC) and adenomas in two independent Norwegian cohorts. Here we seek to verify our previous findings using new cohorts and methods. In addition, we characterize lifestyle and sex specificity, the functional potential of the species, and their interaction with other microbial species. We analyze with 16S rRNA sequencing, shotgun metagenome sequencing, and species-specific qPCR, using 2350 samples from three Norwegian cohorts-CRCAhus, NORCCAP, and CRCbiome-and a large publicly available data set, curatedMetagenomicData. Using metagenome-assembled genomes from the CRCbiome study, we explore the genomic characteristics and functional potential of the pangenome. Three species of associated with adenoma/CRC were consistently detected by qPCR and sequencing. Positive associations with adenomas/CRC were verified for and negative associations were shown for and adenoma in curatedMetagenomicData. Men show a higher prevalence of across cohorts. Co-occurrence among species was low (<6%). Each of the three species shows distinct microbial composition and forms distinct correlation networks with other bacterial taxa, although was negatively correlated to all investigated species. Pangenome analyses showed to be enriched for genes related to porphyrin metabolism and degradation of complex carbohydrates, whereas glycoside hydrolase enzyme 3 was specific to .IMPORTANCEUntil now has been going under the radar as a CRC-associated genus despite having been noted, but overseen, as such for over a decade. We found not just one, but two species of to be associated with CRC- was more abundant in adenoma/CRC, while was less abundant in adenoma. Each of them represents distinct communities, constituted by specific microbial partners and metabolic capacities-and they rarely occur together in the same patients. We have verified that is increased in adenoma and CRC and this species should be recognized among the most important CRC-associated bacteria.
我们之前在两个独立的挪威队列中证明了 与结直肠癌（CRC）和腺瘤的丰度增加之间存在关联。在这里，我们试图使用新的队列和方法来验证我们之前的发现。此外，我们还描述了 与生活方式和性别特异性、 物种的功能潜力及其与其他微生物物种的相互作用。我们使用 16S rRNA 测序、 shotgun 宏基因组测序和物种特异性 qPCR 分析了来自三个挪威队列（CRCAhus、NORCCAP 和 CRCbiome）和一个大型公共可用数据集 curatedMetagenomicData 的 2350 个样本。使用 CRCbiome 研究中的宏基因组组装基因组，我们探索了 的基因组特征和功能潜力。通过 qPCR 和测序一致检测到与腺瘤/CRC 相关的三种 物种。在 curatedMetagenomicData 中，对 与腺瘤/CRC 的正相关关系进行了验证，而对 与腺瘤的负相关关系进行了验证。在所有队列中，男性的 患病率较高。在 物种之间的共现率较低（<6%）。尽管 已经被注意到，但它仍然是一种与 CRC 相关的属，并且被忽视了十多年。尽管如此，这三种物种都显示出不同的微生物组成，并与其他细菌分类群形成不同的相关网络，尽管 与所有被调查的 物种呈负相关。泛基因组分析表明， 与卟啉代谢和复杂碳水化合物降解相关的基因丰富，而糖苷水解酶 3 则是 所特有的。重要的是，尽管 已经被注意到，但它仍然是一种与 CRC 相关的属，并且被忽视了十多年。尽管如此，我们发现不仅有一种，而是两种 物种与 CRC 相关—— 在腺瘤/CRC 中更为丰富，而 在腺瘤中则较少。它们每一种都代表着独特的群落，由特定的微生物伙伴和代谢能力构成——而且它们很少在同一患者中同时出现。我们已经验证了 在腺瘤和 CRC 中增加，这种物种应该被认为是最重要的 CRC 相关细菌之一。
