Hume D A, Allan W, Golder J, Stephens R W, Doe W F, Warren H S
J Leukoc Biol. 1985 Oct;38(4):541-52. doi: 10.1002/jlb.38.4.541.
Bone marrow-derived macrophages were prepared from human bone marrow mononuclear cells following cultivation in GCT-conditioned medium (GCT-CM) and purification by adherence to fibronectin-coated flasks. The growth of bone marrow mononuclear cells in GCT-CM was dependent on the shape of the culture vessels, being increased in round-bottomed versus flat-bottomed wells. Proliferation was confined to nonadherent cells; like blood monocytes, bone marrow-derived macrophages did not incorporate [3H]thymidine in response to GCT-CM or human serum. Purified macrophages from this source expressed nonspecific esterase and OKM1, OKla, FMC 17, 32, and 34 and 25F9 antigens but lacked Mo2. They expressed high levels of an inactivator of plasminogen activator, minactivin, and gave a substantial metabolic burst in response to phorbol myristate acetate or opsonized (but not unopsonized) zymosan. Bone marrow-derived macrophages acted as accessory cells in the response of T lymphocytes to phytohemagglutinin. The results suggest that liquid bone marrow cultures are useful in the study of the differentiation of human mononuclear phagocytes.
从人骨髓单个核细胞制备骨髓来源的巨噬细胞，方法是在GCT条件培养基（GCT-CM）中培养，然后通过贴壁于纤连蛋白包被的培养瓶进行纯化。骨髓单个核细胞在GCT-CM中的生长取决于培养容器的形状，圆底孔中的生长比平底孔中的增加。增殖局限于非贴壁细胞；与血液单核细胞一样，骨髓来源的巨噬细胞不会因GCT-CM或人血清而掺入[3H]胸腺嘧啶核苷。从该来源纯化的巨噬细胞表达非特异性酯酶和OKM1、OKla、FMC 17、32和34以及25F9抗原，但缺乏Mo2。它们表达高水平的纤溶酶原激活物的一种抑制剂——微激活素，并且对佛波酯肉豆蔻酸酯或调理（但非未调理）的酵母聚糖有显著的代谢爆发反应。骨髓来源的巨噬细胞在T淋巴细胞对植物血凝素的反应中起辅助细胞的作用。结果表明，液体骨髓培养在研究人单核吞噬细胞的分化中是有用的。
