Chiang Su Jane Fan, Chien Mei-Kuei, Tsai Chang-Yi, Hsiao Jui-Chang, Koo Fan-Hlan, Yen Yung-Feng, Chou Yi-Chang, Cheng Chih-Chien
Department of Clinical Laboratory, Zhongxiao Branch, Taipei City Hospital, Taipei, Taiwan.
Department of Obstetrics/Gynaecology, Taipei City Hospital, Taipei, Taiwan.
Environ Health Insights. 2024 Sep 14;18:11786302241272398. doi: 10.1177/11786302241272398. eCollection 2024.
Candida is one of the common pathogens in nosocomial infections. Culture is the gold standard for diagnosing candidemia. is identified via the germ tube test, which uses serum as the culture medium, which is costly and time-consuming. This study was conducted to evaluate and compare a relatively simple, fast, and reliable method for the detection of .
We conducted this randomized case study at Taipei City Hospital (TCH) from January 2023 to August 2023, with a total of 30 specimen culture reports collected and confirmed to be cases of infection. A germ tube test was performed in a 37°C water bath using serum, plasma, and safe plasma products (Fresh Frozen Plasma, FFP). Further, the same procedures were repeated with the addition of 22% bovine serum albumin (BSA) to the identification/culture.
By adding BSA, more than 50% of the budding phenomenon was observed in 40 minutes, which shortened the diagnosis time compared with the traditional method (2-3 hours). Using BSA can shorten the identification time for early clinical medication and improve the quality of medical care.
Using safer plasma products for germ tube test of candidiasis not only reduced the risk of infection for medical technicians but could also replace the serum used in traditional methods to increase convenience and save time. This study proposed BSA as a germ tube induction medium enhancer, which reduced the culture time, thereby enabling quicker diagnosis of infections.
念珠菌是医院感染中常见的病原体之一。培养是诊断念珠菌血症的金标准。通过芽管试验进行鉴定，该试验以血清作为培养基，成本高且耗时。本研究旨在评估和比较一种相对简单、快速且可靠的检测方法。
我们于2023年1月至2023年8月在台北市立医院（TCH）开展了这项随机病例研究，共收集了30份标本培养报告并确认为感染病例。在37°C水浴中使用血清、血浆和安全血浆制品（新鲜冰冻血浆，FFP）进行芽管试验。此外，在鉴定/培养中添加22%牛血清白蛋白（BSA）后重复相同步骤。
添加BSA后，40分钟内观察到超过50%的出芽现象，与传统方法（2 - 3小时）相比缩短了诊断时间。使用BSA可缩短鉴定时间以便早期临床用药并提高医疗质量。
使用更安全的血浆制品进行念珠菌病的芽管试验不仅降低了医护人员的感染风险，还可替代传统方法中使用的血清，增加便利性并节省时间。本研究提出将BSA作为芽管诱导培养基增强剂，缩短了培养时间，从而能够更快地诊断感染。
