Hafler D A, Hemler M E, Christenson L, Williams J M, Shapiro H M, Strom T B, Strominger J L, Weiner H L
Clin Immunol Immunopathol. 1985 Nov;37(2):163-71. doi: 10.1016/0090-1229(85)90147-3.
Monoclonal antibodies have recently been characterized which identify activated T cells at different stages of differentiation. We compared the expression of the late appearing activation antigen defined by monoclonal antibody TS2/7 with the expression of early appearing activation antigens in a group of patients with active multiple sclerosis, encephalitis, non-inflammatory other neurologic diseases, and normal controls. An increase in TS2/7 reactivity of peripheral blood T cells was found in MS patients compared to controls (P less than 0.001), however, there was no increase in the level of early activation antigens. This was in contrast to three patients with viral encephalitis, who had an increase in the early activation antigen 4F2, but minimal, if any, increase in the TS2/7 reactive antigen. This study demonstrates that in vivo, as in vitro, it is possible to identify multiple differentiation stages for activated T cells. Furthermore, the presence of activated T cells in the peripheral blood of multiple sclerosis patients suggests that there is systemic immune activation in MS, and could provide a means to monitor abnormal immunologic activity in MS when these cells are functionally characterized.
最近已经鉴定出了一些单克隆抗体，它们能够识别处于不同分化阶段的活化T细胞。我们比较了单克隆抗体TS2/7所定义的晚期出现的活化抗原的表达与一组患有活动性多发性硬化症、脑炎、非炎性其他神经系统疾病的患者以及正常对照中早期出现的活化抗原的表达。与对照组相比，多发性硬化症患者外周血T细胞的TS2/7反应性增加（P小于0.001），然而，早期活化抗原的水平没有增加。这与三名病毒性脑炎患者形成对比，他们的早期活化抗原4F2增加，但TS2/7反应性抗原即使有增加也非常微小。这项研究表明，在体内，如同在体外一样，有可能识别活化T细胞的多个分化阶段。此外，多发性硬化症患者外周血中活化T细胞的存在表明多发性硬化症存在全身性免疫激活，并且当这些细胞的功能特征被确定时，可为监测多发性硬化症中异常的免疫活性提供一种手段。
