Center for Synthetic Biology and Integrated Bioengineering, Westlake University, Hangzhou 310030, Zhejiang, China; School of Engineering, Westlake University, Hangzhou 310030, Zhejiang, China; Zhejiang Provincial Key Laboratory of Intelligent Low-Carbon Biosynthesis, Hangzhou 310030, Zhejiang, China; Research Center for Industries of the Future, Westlake University, No. 600 Dunyu Road, 310030, Zhejiang Province, China.
Guangdong C1 Life Biotech Co., Ltd., Guangzhou 510630, Guangdong, China.; Hua An Tang Biotech Group Co. Ltd., GuangZhou 510630, Guangdong, China.
Biotechnol Adv. 2024 Dec;77:108455. doi: 10.1016/j.biotechadv.2024.108455. Epub 2024 Sep 19.
Diols are important platform chemicals with a wide range of applications in the fields of chemical and pharmaceutical industries, food, feed and cosmetics. In particular, 1,3-propanediol (PDO), 1,4-butanediol (1,4-BDO) and 1,3-butanediol (1,3-BDO) are appealing monomers for producing industrially important polymers and plastics. Therefore, the commercialization of bio-based diols is highly important for supporting the growth of biomanufacturing for the fiber industry. This review focuses primarily on the microbial production of PDO, 1,4-BDO and 1,3-BDO with respect to different microbial strains and biological routes. In addition, metabolic platforms which are designed to produce various diols using generic bioconversion strategies are reviewed for the first time. Finally, we also summarize and discuss recent developments in the downstream processing of PDO according to their advantages and drawbacks, which is taken as an example to present the prospects and challenges for industrial separation and purification of diols from microbial fermentation broth.
二醇是重要的平台化学品，在化学和制药、食品、饲料和化妆品等领域有广泛的应用。特别是 1,3-丙二醇（PDO）、1,4-丁二醇（1,4-BDO）和 1,3-丁二醇（1,3-BDO）是用于生产具有工业重要性的聚合物和塑料的有吸引力的单体。因此，生物基二醇的商业化对于支持纤维工业的生物制造的增长非常重要。本文主要关注不同微生物菌株和生物途径的微生物生产 PDO、1,4-BDO 和 1,3-BDO。此外，还首次综述了使用通用生物转化策略设计用于生产各种二醇的代谢平台。最后，根据各自的优缺点，我们还总结和讨论了 PDO 的下游加工的最新进展，以其为例展示了从微生物发酵液中分离和纯化二醇的工业分离和纯化的前景和挑战。
