Demir Burak Furkan, Topcuoglu Canan, Turhan Turan, Altıparmak Emin, Yılmaz Nisbet, Ateş İhsan
Ankara City Hospital, Internal Medicine Department, Ankara, Turkey.
Ankara City Hospital, Department of Biochemistry, Ankara, Turkey.
Gastroenterol Hepatol Bed Bench. 2024;17(3):270-278. doi: 10.22037/ghfbb.v17i3.2968.
This study aimed to compare dynamic thiol/disulfide homeostasis and myeloperoxidase (MPO) levels in patients with Gilbert's syndrome (GS) and healthy controls.
Thiol/disulfide homeostasis and MPO levels are both associated with increased progression of atherosclerosis.
The study included a total of 130 voluntary participants comprising 65 patients with GS and 65 healthy controls. These patients were selected randomly and dynamic thiol/disulfide homeostasis, MPO, complete blood count results, and biochemistry and lipid parameters were evaluated. Patients with known chronic diseases, medication usage, and acute infections were excluded from the study. Serum total thiol and native thiol levels were measured using the fully automated colorimetric method, while serum MPO levels were measured using the sandwich ELISA method.
We found that patients with GS had significantly higher total thiol (352.3±38.6 vs. 317.9±47.9, p<0.001) and native thiol (386.6±42.6 vs. 348.0±51.1, p<0.001) and significantly lower disulfide (15.7±4.0 vs. 17.3±4.0, p=0.022) and MPO (130.7 vs. 166.3, p=0.006). In patients with bilirubin of <1 mg/dL, total thiol and native thiol levels were lower and disulfide, disulfide/native thiol (DNT) and disulfide/total thiol (DTT) ratios, and MPO levels were higher. Patients with bilirubin of <1 mg/dL also had higher total cholesterol.
In these patients with GS, the thiol/disulfide balance shifted towards thiols and proinflammatory MPO levels were lower. When bilirubin was <1 mg/dL, disulfide, DNT and DTT ratios, and MPO were higher. Bilirubin levels affected all parameters of thiol/disulfide homeostasis and MPO levels independently of other risk factors. In light of our results, we suggest that mild hyperbilirubinemia in cases of GS has an anti-inflammatory and antioxidant effect and may be protective against atherosclerosis.
本研究旨在比较吉尔伯特综合征（GS）患者与健康对照者的动态硫醇/二硫键稳态及髓过氧化物酶（MPO）水平。
硫醇/二硫键稳态和MPO水平均与动脉粥样硬化进展加快有关。
本研究共纳入130名自愿参与者，其中包括65例GS患者和65名健康对照者。这些患者被随机选取，对其动态硫醇/二硫键稳态、MPO、全血细胞计数结果以及生化和血脂参数进行评估。已知患有慢性疾病、正在使用药物以及患有急性感染的患者被排除在研究之外。血清总硫醇和天然硫醇水平采用全自动比色法测量，而血清MPO水平采用夹心酶联免疫吸附测定法测量。
我们发现，GS患者的总硫醇（352.3±38.6对317.9±47.9，p<0.001）和天然硫醇（386.6±42.6对348.0±51.1，p<0.001）显著更高，而二硫键（15.7±4.0对17.3±4.0，p=0.0 [原文有误，应为0.022]）和MPO（130.7对166.3，p=0.006）显著更低。在胆红素<1mg/dL的患者中，总硫醇和天然硫醇水平较低，而二硫键、二硫键/天然硫醇（DNT）和二硫键/总硫醇（DTT）比值以及MPO水平较高。胆红素<1mg/dL的患者总胆固醇也更高。
在这些GS患者中，硫醇/二硫键平衡向硫醇方向偏移，促炎MPO水平较低。当胆红素<1mg/dL时，二硫键、DNT和DTT比值以及MPO更高。胆红素水平独立于其他危险因素影响硫醇/二硫键稳态和MPO水平所有参数。根据我们的研究结果，我们认为GS患者的轻度高胆红素血症具有抗炎和抗氧化作用，可能对动脉粥样硬化具有保护作用。
