Massarrat S
Hepatogastroenterology. 1985 Aug;32(4):185-90.
Serum pepsin activity was measured in 359 patients with various gastric diseases, the serum protein themselves serving as substrate. In 332 out of 359 patients, BAO and PAO, and in 173 of these patients, pepsin activity, were determined in gastric juices. In 102 patients, serum pepsin activity was measured before, and 45 and 90 minutes after, pentagastrin stimulation. In 71 out of these 102 patients, pepsinogen I was measured simultaneously by RIA. There was good correlation between stimulated serum pepsin activity and maximal pepsin output (r = 0.63), as well as BAO (r = 0.67) and PAO (r = 0.77). The higher the PAO, the greater the increase in pepsin activity in the sera after pentagastrin stimulation. The increase in pepsinogen I under stimulation was smaller in the sera than pepsin activity. There was a slightly better correlation of PAO with stimulated serum pepsin activity than with pepsinogen I (r = 0.76 versus 0.62). The measurement of pentagastrin-stimulated pepsin activity in sera, as a very simple and inexpensive method, permits the screening, with an overall accuracy of more than 75%, of achlorhydrics (stimulated pepsin activity less than 30 micrograms thyrosine/24 hours/ml serum), acid secretors (greater than 80 micrograms thyrosine/24 hours/ml serum) and hypersecretors (PAO greater than 35 mmol HCl/hours, greater than 120 micrograms thyrosine/24 hours/ml serum).
以血清蛋白自身作为底物，测定了359例患有各种胃部疾病患者的血清胃蛋白酶活性。在359例患者中的332例测定了胃液中的基础胃酸分泌量（BAO）和最大胃酸分泌量（PAO），其中173例患者测定了胃液中的胃蛋白酶活性。在102例患者中，测定了五肽胃泌素刺激前、刺激后45分钟和90分钟的血清胃蛋白酶活性。在这102例患者中的71例，同时用放射免疫分析法测定了胃蛋白酶原I。刺激后的血清胃蛋白酶活性与最大胃蛋白酶分泌量（r = 0.63）、BAO（r = 0.67）和PAO（r = 0.77）之间存在良好的相关性。PAO越高，五肽胃泌素刺激后血清中胃蛋白酶活性的增加就越大。刺激后血清中胃蛋白酶原I的增加量比胃蛋白酶活性小。PAO与刺激后的血清胃蛋白酶活性的相关性略优于与胃蛋白酶原I的相关性（r = 0.76对0.62）。作为一种非常简单且廉价的方法，测定五肽胃泌素刺激后的血清胃蛋白酶活性，对无胃酸分泌者（刺激后的胃蛋白酶活性低于30微克酪氨酸/24小时/毫升血清）、胃酸分泌者（高于80微克酪氨酸/24小时/毫升血清）和胃酸分泌过多者（PAO高于35毫摩尔盐酸/小时，高于120微克酪氨酸/24小时/毫升血清）进行筛查的总体准确率超过75%。
