Vianello F, Di Mario F, Plebani M, Germana B, Dal Santo P, Leandro G, Dotto P, Grassi S A, Battaglia G, Naccarato R
Istituto di Medicina Interna, Università Degli Studi di Padova, Italy.
Dig Dis Sci. 1994 Feb;39(2):301-8. doi: 10.1007/BF02090201.
A modification of Berstad's spectrophotometric method was tested and proved capable of detecting pepsin concentrations in mucosal perendoscopic biopsy homogenates. The relationship between this parameter and pepsin in gastric juice and pepsinogen group I in serum and in biopsy homogenates was analyzed. From the biochemical point of view, the assay was found sufficiently accurate. Mucosal pepsinogen group I, but not mucosal pepsin, concentration was found higher in gastric and duodenal ulcer patients than in controls. Patients with corpus-fundic gastric ulcer showed significantly lower mucosal pepsin and mucosal pepsinogen group (PG) I. Aging and smoking did not influence either parameter but male duodenal ulcer subjects presented higher mucosal pepsinogen group I concentration. The lack of any relationship between serum and mucosal PG I and between pepsin in gastric juice and in mucosa raises a question, at least in methodological terms, about the validity of using serum pepsinogen group I and pepsin as indicators of peptic output.
对Berstad分光光度法进行了改良并进行测试，结果证明该方法能够检测经内镜活检黏膜匀浆中的胃蛋白酶浓度。分析了该参数与胃液中的胃蛋白酶以及血清和活检匀浆中I型胃蛋白酶原之间的关系。从生化角度来看，该检测方法足够准确。发现胃溃疡和十二指肠溃疡患者的黏膜I型胃蛋白酶原浓度高于对照组，但黏膜胃蛋白酶浓度并非如此。胃体-胃底溃疡患者的黏膜胃蛋白酶和黏膜I型胃蛋白酶原（PG）浓度显著较低。年龄增长和吸烟对这两个参数均无影响，但男性十二指肠溃疡患者的黏膜I型胃蛋白酶原浓度较高。血清和黏膜PG I之间以及胃液和黏膜中的胃蛋白酶之间缺乏任何关联，这至少在方法学方面对将血清I型胃蛋白酶原和胃蛋白酶用作消化液分泌指标的有效性提出了质疑。
