吉尔吉斯斯坦进口驴中[具体物质]的首个分子证据。 （原文中“and”后面似乎缺失了关键信息）
First Molecular Evidence of and in Imported Donkeys from Kyrgyzstan.
作者信息
Wu Xuanchen, Xu Jun, Su Lixin, Li Ente, Wang Suwen, Hornok Sándor, Liu Gang, Wang Yuanzhi
机构信息
NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China.
Urumqi Customs, Urumqi 833400, China.
出版信息
Pathogens. 2024 Aug 23;13(9):713. doi: 10.3390/pathogens13090713.
Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi, Theileria haneyi, and Babesia caballi. Nonetheless, there has been a scarcity of systematic reports on EP parasites in donkeys in Kyrgyzstan, Central Asia. In this study, piroplasms were screened in 1900 blood samples from imported donkeys from the Osh Oblast (southwestern Kyrgyzstan) by targeting partial 18S ribosomal RNA using the polymerase chain reaction (PCR). Through molecular and phylogenetic analyses, all positive samples were sequenced to identify the species and genotypes. The results indicated the presence of both B. caballi and T. equi, with prevalence rates of 8.4% (160/1900) and 12.2% (232/1900), respectively. By amplifying part of the Erythrocyte Merozoite Antigen 1 (EMA-1) and Rhoptry-Associated Protein (RAP-1) genes, B. caballi genotype B and T. equi genotype A were identified. To the best of our knowledge, this is the first report on piroplasm infection among donkeys from Kyrgyzstan.
马焦虫病（EP）是马属动物的一种重要的蜱传疾病，由马泰勒虫、哈尼泰勒虫和马巴贝斯虫引起。然而，在中亚吉尔吉斯斯坦，关于驴的马焦虫病寄生虫的系统报告却很匮乏。在本研究中，通过聚合酶链反应（PCR）靶向部分18S核糖体RNA，对来自吉尔吉斯斯坦西南部奥什州的1900份进口驴血样进行了焦虫筛查。通过分子和系统发育分析，对所有阳性样本进行测序以鉴定其种类和基因型。结果表明，马巴贝斯虫和马泰勒虫均有存在，流行率分别为8.4%（160/1900）和12.2%（232/1900）。通过扩增部分红细胞裂殖子抗原1（EMA-1）和棒状体相关蛋白（RAP-1）基因，鉴定出马巴贝斯虫基因型B和马泰勒虫基因型A。据我们所知，这是关于吉尔吉斯斯坦驴焦虫感染的首次报告。
Zotero 插件