二硫化秋兰姆与二硫代氨基甲酸盐之间的交叉反应性。使用小鼠模型对双硫仑和代森锌的研究。
Cross-reactivity between thiuram disulfides and dithiocarbamates. A study of TETD and ZDEC using mouse models.
作者信息
Kursawe Larsen Christoffer, Funch Anders Boutrup, Vaher Helen, Lohmann Rebecca Kitt Davidson, Jee Mia Hamilton, Schwensen Jakob F B, Zachariae Claus, Svedman Cecilia, Bergendorff Ola, Bonefeld Charlotte Menné, Johansen Jeanne D
机构信息
National Allergy Research Centre, Department of Dermatology and Allergy, Herlev and Gentofte Hospital, Hellerup, Denmark.
Institute of Clinical Medicine, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark.
出版信息
Contact Dermatitis. 2025 Feb;92(2):137-144. doi: 10.1111/cod.14706. Epub 2024 Sep 28.
BACKGROUND
Rubber accelerators are used in the vulcanization of rubber. However, rubber accelerators for example tetraethylthiuram disulfide (TETD) and zinc diethyldithiocarbamate (ZDEC) may cause contact allergy. Concomitant reactions between ZDEC and TETD have been observed in patients which could be explained by co- or cross-reactivity.
OBJECTIVES
To investigate cross-reactivity between TETD and ZDEC and vice versa.
METHODS
Groups of mice were sensitized with TETD or ZDEC based on reported EC3-values. Proliferation of lymphocytes were measured on day 5. To test cross-reactivity, mice were sensitized and challenged 3 weeks later with TETD or ZDEC. The inflammatory response was measured by changes in ear thickness and the proliferative response in CD4 and CD8 T cells in the submandibular and cervical draining lymph nodes.
RESULTS
Sensitization of mice with doses of ZDEC 3%, TETD 5.6% or TETD 16.2% induced significant increased ear thickness and proliferation of CD4 and CD8 T cells. Challenge with ZDEC or TETD in these groups induced significant increased ear thickness. Challenge with ZDEC in mice sensitized to TETD 5.6% or TETD 16.2% induced significant increased proliferation of CD4 and CD8 T cells.
CONCLUSIONS
We show cross-reactivity between TETD and ZDEC. Patients sensitized to TETD or ZDEC should avoid exposure to both ZDEC and TETD.
背景
橡胶促进剂用于橡胶的硫化过程。然而，橡胶促进剂如二硫化四乙基秋兰姆（TETD）和二乙基二硫代氨基甲酸锌（ZDEC）可能会引起接触性过敏。在患者中已观察到ZDEC和TETD之间的伴随反应，这可能由共同反应性或交叉反应性来解释。
目的
研究TETD和ZDEC之间的交叉反应性，反之亦然。
方法
根据报告的EC3值，用TETD或ZDEC对几组小鼠进行致敏。在第5天测量淋巴细胞的增殖情况。为了测试交叉反应性，对小鼠进行致敏，并在3周后用TETD或ZDEC进行激发。通过耳部厚度的变化以及下颌下和颈部引流淋巴结中CD4和CD8 T细胞的增殖反应来测量炎症反应。
结果
用3%的ZDEC、5.6%的TETD或16.2%的TETD对小鼠进行致敏，可导致耳部厚度显著增加以及CD4和CD8 T细胞增殖。用ZDEC或TETD对这些组进行激发可导致耳部厚度显著增加。用ZDEC对致敏于5.6%或16.2% TETD的小鼠进行激发，可导致CD4和CD8 T细胞增殖显著增加。
结论
我们证明了TETD和ZDEC之间存在交叉反应性。对TETD或ZDEC致敏的患者应避免接触ZDEC和TETD两者。
Zotero 插件