Binh Hoang Thi, Diep Trinh Thi, Van Ngoc Nguyen
Faculty of Biology, Dalat University, Dalat, Lam Dong, 670000, Vietnam.
Faculty of Chemistry and Environment, Dalat University, Dalat, Vietnam.
Chem Biodivers. 2025 Feb;22(2):e202401865. doi: 10.1002/cbdv.202401865. Epub 2024 Nov 8.
The essential oil from the leaves of Blumea densiflora var. hookeri was analyzed using GC-MS, and bioassays were performed. GC-MS identified thirty-four constituents, representing 79.1 % of the oil composition. The major compounds identified in the oil were (E)-caryophyllene (15.3 %), viridiflorol (5.5 %), trans-dauca-4(11),7-diene (4.6 %), spathulenol (4.4 %), and isolongifolan-7-α-ol (4.3 %). The antimicrobial activity of the oil was assessed to determine its efficacy against six bacterial strains and one fungal strain using minimum inhibitory concentration (MIC) methods. The essential oil demonstrated antimicrobial activity against all tested microorganisms. A cytotoxicity assay was performed on KB and A549 cancer cell lines using the MTT method. The essential oil displayed strong cytotoxic effects on both cell lines, with IC values of 1.40±0.05 μg/mL for KB and 1.72±0.04 μg/mL for A549.
采用气相色谱-质谱联用仪（GC-MS）对密花艾纳香（Blumea densiflora var. hookeri）叶片的精油进行了分析，并进行了生物测定。GC-MS鉴定出34种成分，占精油成分的79.1%。精油中鉴定出的主要化合物为（E）-石竹烯（15.3%）、绿花白千层醇（5.5%）、反式-胡萝卜-4(11),7-二烯（4.6%）、匙叶桉油烯醇（4.4%）和异长叶烯-7-α-醇（4.3%）。采用最低抑菌浓度（MIC）法评估了该精油对6种细菌菌株和1种真菌菌株的抗菌活性，以确定其疗效。该精油对所有测试微生物均表现出抗菌活性。采用MTT法对KB和A549癌细胞系进行了细胞毒性试验。该精油对两种细胞系均表现出强烈的细胞毒性作用，KB细胞系的IC值为1.40±0.05μg/mL，A549细胞系的IC值为1.72±0.04μg/mL。
