Green Synthesis of Copper Nanoparticles Using Roots and their Bioactivities Against , and .

The synthesis of copper nanoparticles (CuNPs) has been the center of researchers' attention due to their potential in various fields, particularly in medicine and pharmacy. The objective of this research was to synthesize CuNPs by using the extract from longifolia roots via an organic solvent-free approach. The roots of were extracted using boiling water and subsequently used to synthesize CuNPs from CuSO solution. The resultant nanoparticles were then characterized using UV-Vis spectrophotometer, (FTIR), (XRD), (SEM-EDX), and (TEM). Kirby-Bauer sensitivity test was performed against , , and . In the results, we found that the total phenolic content and total flavonoid content of the extract were 85.43 GAE/g dry extract and 0.78 QE/g dry extract, respectively. The characterizations revealed that the obtained nanoparticles possess a spherical shape and diameters of around 2 to 10 nm. The XRD suggests that the nanoparticles were occupied by copper (0) and copper oxides (CuO, CuO, and CuO). The synthesized CuNPs yielded inhibition zones of 17.2 ± 0.2, 15.6 ± 0.2, and 13.7 ± 0.1 mm against , , and , respectively. In conclusion, aqueous extract from the roots of can be used to synthesize CuNPs that could be used as an antibacterial and antifungal agent.