Purnomo A T, Syarif A N, Wardhana A, Siregar N C
Plastic, Reconstructive and Aesthetic Surgery Division, Department of Surgery, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Anatomical Pathology Department, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Ann Burns Fire Disasters. 2024 Sep 30;37(3):242-249. eCollection 2024 Sep.
Burn injury remains a health problem, specifically in Indonesia. In major burns, xenograft has been proved to be useful as temporary wound coverage. However, some xenografts are not widely available due to cultural, financial and religious backgrounds, or have an unesthetic appearance, such as the scaly appearance of tilapia fish xenograft. Striped catfish () is a scaleless fish that has abundant type 1 collagen. This study aimed to compare striped catfish skin to commonly used xenograft (Nile tilapia and porcine skin) as xenograft material for burn wound. In this experimental study, nine different skin samples of striped catfish, Nile tilapia and porcine were prepared and histologically examined using hematoxylin-eosin stained samples. Macroscopic and microscopic features of each sample were documented and analysed. The macroscopic skin appearances of striped catfish were hairless and scaleless with black-silver color and moderate thickness. As for microscopic features, the epidermal thickness of striped catfish skin (8.49±1.60 μm) was significantly different to both Nile tilapia (2.18±0.37 μm; p<0.001) and porcine skin (42.22±14.85 μm; p=0.002). The dermal thickness of striped catfish skin (288.46±119.04 μm) was similar to Nile tilapia (210.68±46.62 μm; p=0.783) but differs significantly to porcine skin (1708.44±505.12 μm; p<0.001). The integrity and collagen organization of striped catfish was also similar to tilapia based on semi-quantitative histology scoring system (p>0.05). Striped catfish had potential macroscopic appearance and comparable microscopic features to Nile tilapia; smoother macroscopic appearance, thicker epidermis, and similar dermis thickness. Therefore, we believe it can be potentially used as a xenograft material. Further studies are required to evaluate the effectiveness and feasibility of striped catfish xenograft in burn wound management.
烧伤仍是一个健康问题，在印度尼西亚尤为如此。在大面积烧伤中，异种移植物已被证明可用作临时伤口覆盖物。然而，由于文化、经济和宗教背景，一些异种移植物无法广泛获取，或者外观不佳，比如罗非鱼异种移植物的鳞片外观。条纹鲶鱼是一种无鳞鱼，含有丰富的I型胶原蛋白。本研究旨在比较条纹鲶鱼皮与常用异种移植物（尼罗罗非鱼皮和猪皮）作为烧伤创面异种移植物材料的情况。在这项实验研究中，制备了九种不同的条纹鲶鱼、尼罗罗非鱼和猪的皮肤样本，并使用苏木精-伊红染色样本进行组织学检查。记录并分析每个样本的宏观和微观特征。条纹鲶鱼的宏观皮肤外观无毛、无鳞，呈黑银色，厚度适中。至于微观特征，条纹鲶鱼皮肤的表皮厚度（8.49±1.60μm）与尼罗罗非鱼（2.18±0.37μm；p<0.001）和猪皮（42.22±14.85μm；p=0.002）均有显著差异。条纹鲶鱼皮肤的真皮厚度（288.46±119.04μm）与尼罗罗非鱼（210.68±46.62μm；p=0.783）相似，但与猪皮（1708.44±505.12μm；p<0.001）有显著差异。基于半定量组织学评分系统，条纹鲶鱼的完整性和胶原蛋白组织也与罗非鱼相似（p>0.05）。条纹鲶鱼具有与尼罗罗非鱼相似的潜在宏观外观和可比的微观特征；宏观外观更光滑，表皮更厚，真皮厚度相似。因此，我们认为它有可能用作异种移植物材料。需要进一步研究来评估条纹鲶鱼异种移植物在烧伤创面处理中的有效性和可行性。
