Kodgi Venkatesh, Shetty Priya, Thimmaiah Charisma, Thomas Nithya Annie, Vergis Bettina Ashwini, Shetty Kaushik
Department of Pediatric and Preventive Dentistry, Pearl Dental Speciality Centre, Muscat, Oman.
Department of Pediatric and Preventive Dentistry, A. J. Institute of Dental Sciences, Mangaluru, Karnataka, India.
Int J Clin Pediatr Dent. 2024 May;17(5):591-595. doi: 10.5005/jp-journals-10005-2875.
and are the chief microbes associated with severe early childhood caries (S-ECC). Diverse antimicrobial agents are widely used to prevent ECC, and a quest for newer natural products has been on the rise in the recent past.
To estimate the antimicrobial activity of propolis with chlorhexidine on salivary specimens from children with S-ECC .
A total of 60 children with S-ECC were designated. Salivary samples of 30 children (group I) were inoculated onto mitis salivarius agar (MSA) to isolate . Another 30 samples (group II) were inoculated on sabouraud's dextrose agar and subcultured on HiCrome differential agar to isolate . Sensitivity testing for 0.2% chlorhexidine and 10% propolis extract was done using the agar well diffusion technique using Mueller-Hinton agar medium. The antimicrobial effect was evaluated by calculating the diameter of the zone of inhibition surrounding the well.
All saliva samples collected from groups I and II showed growth of and , respectively. All cultured microbes were sensitive to 0.2% chlorhexidine and 10% propolis extract. The mean inhibition zone for with chlorhexidine was 14.57 ± 0.63 mm, and with propolis, 11.93 ± 0.52 mm. The mean zone of inhibition for with chlorhexidine was 12.83 ± 0.59 mm, and with propolis, 9.50 ± 0.73 mm. Chlorhexidine consistently showed statistically significantly larger zones of inhibition and hence appeared to be a more potent antimicrobial agent than propolis extract for both and . However, propolis has irrefutable action against both and .
Propolis may be an acceptable substitute for chlorhexidine for long-term use as it has demonstrated antimicrobial activity and fewer side effects. Hence, this Association of Physicians of India herbal drug can be incorporated into mouthwashes and toothpaste to reduce microbial counts.
Kodgi V, Shetty P, Thimmaiah C, Comparative Assessment of Antimicrobial Activity of Propolis and Chlorhexidine on Salivary Isolates of and in Children with Severe Early Childhood Caries: An Study. Int J Clin Pediatr Dent 2024;17(5):591-595.
变形链球菌和远缘链球菌是与重度幼儿龋（S-ECC）相关的主要微生物。多种抗菌剂被广泛用于预防幼儿龋，并且最近对新型天然产物的探索一直在增加。
评估蜂胶与氯己定对重度幼儿龋患儿唾液标本的抗菌活性。
共指定60名重度幼儿龋患儿。将30名儿童（第一组）的唾液样本接种到唾液链球菌琼脂（MSA）上以分离变形链球菌。另外30个样本（第二组）接种在沙氏葡萄糖琼脂上，并在HiCrome鉴别琼脂上进行传代培养以分离远缘链球菌。使用穆勒-欣顿琼脂培养基，采用琼脂孔扩散技术对0.2%氯己定和10%蜂胶提取物进行敏感性测试。通过计算孔周围抑菌圈的直径来评估抗菌效果。
从第一组和第二组收集的所有唾液样本分别显示出变形链球菌和远缘链球菌的生长。所有培养的微生物对0.2%氯己定和10%蜂胶提取物均敏感。氯己定对变形链球菌的平均抑菌圈为14.57±0.63毫米，蜂胶为11.93±0.52毫米。氯己定对远缘链球菌的平均抑菌圈为12.83±0.59毫米，蜂胶为9.50±0.73毫米。氯己定始终显示出在统计学上显著更大的抑菌圈，因此对于变形链球菌和远缘链球菌而言，它似乎是比蜂胶提取物更有效的抗菌剂。然而，蜂胶对变形链球菌和远缘链球菌都有确凿的作用。
蜂胶可能是氯己定长期使用的可接受替代品，因为它已证明具有抗菌活性且副作用较少。因此，这种印度医师协会草药药物可纳入漱口水和牙膏中以减少微生物数量。
Kodgi V, Shetty P, Thimmaiah C, 重度幼儿龋患儿唾液中变形链球菌和远缘链球菌分离株的蜂胶与氯己定抗菌活性的比较评估：一项体外研究。《国际临床儿科牙科学杂志》2024；17(5):591-595。
