Guo Jingqiao, Xin Jianhui, Wang Jine, Li Zhen, Yang Jianlei, Yu Xue, Yan Mengxia, Mo Jiangyang
College of Medicine and Nursing, Shandong Engineering Research Center of Novel Pharmaceutical Excipients, Sustained and Controlled Release Preparations, Dezhou University, Dezhou, 253023, China.
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.
Sci Rep. 2024 Oct 2;14(1):22918. doi: 10.1038/s41598-024-74411-7.
Tetracyclines (TCs) rank second globally in the use of animal infection therapy and animal husbandry as growth promoters among all antibiotics. However, large amounts of TCs residue in food products and more than 75% of TCs are excreted into the environment, causing adverse effects on the ecological system and human health. It has been challenging to simultaneously realize low-cost, rapid, and highly selective detection of TCs. Here, inspired by the fluorogenic reactions between resorcinol and catecholamines, we find the fluorescence quenching ability of tetracycline (TC) and firstly propose a fluorescent "turn-off" detection of TC using dopamine and 4-fluororesorcinol. The optimal reaction condition for the fluorescent assay is investigated and the optimized probe showed a good limit of detection (LOD of 1.7 µM) and a wide linear range (10 µM to 350 µM). Moreover, this fluorescent assay proved to be an effective tool for detecting TC in river, Sprite, and beer samples, which represent the aquatic environments and food and may contain tetracyclines residues. Finally, the high selectivity of the method for TC has been confirmed by eliminating the interference from common substances. The proposed strategy provides a high-efficiency and selective solution for the detection of TCs in environment and food and the application fields of this fluorescent assay could be further expanded in the future.
在所有抗生素中，四环素（TCs）在动物感染治疗和作为生长促进剂用于畜牧业方面的使用量在全球排名第二。然而，大量的四环素残留在食品中，超过75%的四环素被排放到环境中，对生态系统和人类健康造成不利影响。同时实现低成本、快速且高选择性地检测四环素一直具有挑战性。在此，受间苯二酚与儿茶酚胺之间荧光反应的启发，我们发现了四环素（TC）的荧光猝灭能力，并首次提出使用多巴胺和4-氟间苯二酚对四环素进行荧光“关断”检测。研究了荧光测定的最佳反应条件，优化后的探针显示出良好的检测限（检测限为1.7 μM）和较宽的线性范围（10 μM至350 μM）。此外，这种荧光测定方法被证明是检测河水、雪碧和啤酒样品中四环素的有效工具，这些样品代表了水生环境和食品，可能含有四环素残留。最后，通过消除常见物质的干扰，证实了该方法对四环素具有高选择性。所提出的策略为环境和食品中四环素的检测提供了一种高效且选择性的解决方案，这种荧光测定方法的应用领域在未来可能会进一步扩大。
