中国河北一起轮状病毒 C 引起的急性肠胃炎爆发的基因组分析。
Genomic analysis of an acute gastroenteritis outbreak caused by rotavirus C in Hebei, China.
机构信息
Chinese PLA Center for Disease Control and Prevention, 20 Dongda Street, Fengtai District, Beijing, 100071, China.
Tianjin Binhai New Area Center for Disease Control and Prevention, Tianjin, 300450, China.
出版信息
Virol J. 2024 Oct 2;21(1):242. doi: 10.1186/s12985-024-02486-9.
Rotavirus group C is an important cause of sporadic cases and outbreaks of gastroenteritis worldwide. Whole-Genome sequences of human rotavirus C (RVC) in public databases are limited. We performed genome sequencing to analyze a RVC outbreak of acute gastroenteritis in China. Samples from 22 patients were screened for pathogens using RT-PCR, and six samples were positive for rotavirus. Whole-Genome sequencing analysis showed that the outbreak strain SJZ217 belongs to the G4-P[2]-I2-R2-C2-M3-A2-N2-T2-E2-H2 genotype and shares almost identical genomic sequences with Chungnam isolated in Korea. Phylogenetic analysis revealed strain SJZ217 also fell into a cluster with rotavirus C strains from Japan and Europe. Reassortment in the VP4 fragment was observed. These results helped to understand the genetic diversity and possible spread of RVC strains.
轮状病毒 C 组是引起全球散发性和暴发胃肠炎的重要病原体。公共数据库中人类轮状病毒 C（RVC）的全基因组序列有限。我们进行了基因组测序，以分析中国一起 RVC 暴发的急性胃肠炎。使用 RT-PCR 对 22 例患者的样本进行了病原体筛查，其中 6 例样本检测出轮状病毒阳性。全基因组测序分析显示，暴发株 SJZ217 属于 G4-P[2]-I2-R2-C2-M3-A2-N2-T2-E2-H2 基因型，与韩国分离的 Chungnam 株具有几乎完全相同的基因组序列。系统进化分析显示，SJZ217 株也与来自日本和欧洲的轮状病毒 C 株聚类在一起。在 VP4 片段观察到重组。这些结果有助于了解 RVC 株的遗传多样性和可能的传播。
Zotero 插件