State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, PR China.
National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun, 130012, PR China.
Biosens Bioelectron. 2025 Jan 1;267:116819. doi: 10.1016/j.bios.2024.116819. Epub 2024 Sep 30.
Membrane proteins are involved in a variety of dynamic cellular processes and exploration of the structural basis of membrane proteins is of significance for a better understanding of their functions. In situ analysis of membrane proteins and their dynamics is, however, challenging for conventional techniques. Surface-enhanced Raman spectroscopy (SERS) is powerful in protein structural characterization, allowing for sensitive, in-situ and real-time identification and dynamic monitoring under physiological conditions. In this review, the applications of SERS in probing membrane proteins are outlined, discussed and prospected. It starts with a brief introduction to membrane proteins, SERS theories and SERS-based strategies that commonly-used for membrane proteins. How to assemble phospholipid biolayers on SERS-active materials is highlighted, followed by respectively discussing about direct and indirect strategies for membrane protein sensing. SERS-based monitoring of protein-ligand interactions is finally introduced and its potential in biomedical applications is discussed in detail. The review ends with critical discussion about current challenges and limitations of this research field, and the promising perspectives in both fundamental and applied sciences.
膜蛋白参与多种动态细胞过程，探索膜蛋白的结构基础对于更好地理解其功能具有重要意义。然而，传统技术对膜蛋白及其动力学的原位分析具有挑战性。表面增强拉曼光谱（SERS）在蛋白质结构表征方面具有强大的功能，能够在生理条件下进行敏感、原位和实时的识别和动态监测。本文综述了 SERS 在探测膜蛋白方面的应用，并对其进行了讨论和展望。本文首先简要介绍了膜蛋白、SERS 理论和基于 SERS 的常用于膜蛋白的策略。接着，重点介绍了如何在 SERS 活性材料上组装磷脂双层，然后分别讨论了直接和间接的膜蛋白传感策略。最后介绍了基于 SERS 的蛋白质-配体相互作用监测，并详细讨论了其在生物医学应用中的潜力。本文最后对该研究领域当前的挑战和局限性进行了批判性讨论，并对基础科学和应用科学的有前途的前景进行了展望。
