Mosleh Ahmed T, Kamoun Elbadawy A, El-Moslamy Shahira H, Salim Samar A, Zahran Heba Y, Zyoud Samer H, Yahia Ibrahim S
Nanotechnology Section, Egyptian Company for Carbon Materials, El-Sheraton/El-Nozha, 11757, Cairo, Egypt.
Department of Chemistry, College of Science, King Faisal University, 31982, Al-Ahsa, Saudi Arabia.
Discov Nano. 2024 Oct 5;19(1):166. doi: 10.1186/s11671-024-04108-3.
The auto-combustion method synthesized CuO NPs and Ag/CuO NPs. The Ag/CuO NPs were analyzed using Fourier-transform infrared, X-ray diffraction, scanning electron microscope, and Energy-dispersive X-ray spectroscopy instrumental analyses. The energy band gap, as determined by DRS properties, decreases from 3.82 to 3.50 eV for pure CuO and 10% Ag/CuO NPs, respectively. The photodegradation efficiency of Rhodamine-B & Carmine by 10% Ag/CuO NPs was nearly 98.9 and 97.8%, respectively. Antimicrobial trials revealed that the antimicrobial efficacy of Ag/CuO NPs at several dosages (20, 40, 60, 80, 100, and 120 µg/mL) against human pathogens was initially assessed using the agar well-diffusion method, and then the broth dilution method. Noticeably, the minimum inhibitory concentration of Ag/CuO NPs for all pathogens ranged from 100 to 120 µg/ml, was determined. Generally, the observed minimum microbicide concentration has a wide range of Ag/CuO NPs doses, ranging from 150 to 300 µg/ml, which helps kill (99.99%) all tested pathogenic cells. The largest relative inhibitory activities (%) were recorded against Escherichia coli (81.45 ± 1.39) at 120 g/mL of Ag/CuO NPs and 100 μg/mL (80.43 ± 0.59), followed by 80 µg/mL (72.33 ± 0.82). Additionally, the lowest relative inhibitory activities (%) were monitored versus fungal cells and Gram-positive bacteria at 120 µg/mL of Ag/CuO NPs as 52.17 ± 1.49 and 53.42 ± 1.71; respectively.
采用自动燃烧法合成了氧化铜纳米颗粒（CuO NPs）和银/氧化铜纳米颗粒（Ag/CuO NPs）。利用傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和能量色散X射线光谱仪对Ag/CuO NPs进行了分析。通过漫反射光谱（DRS）特性测定的能带隙，纯CuO和10% Ag/CuO NPs分别从3.82 eV降至3.50 eV。10% Ag/CuO NPs对罗丹明B和胭脂红的光降解效率分别接近98.9%和97.8%。抗菌试验表明，首先使用琼脂扩散法，然后使用肉汤稀释法，对几种剂量（20、40、60、80、100和120μg/mL）的Ag/CuO NPs对人类病原体的抗菌效果进行了初步评估。值得注意的是，已确定Ag/CuO NPs对所有病原体的最低抑菌浓度范围为100至120μg/ml。一般来说，观察到的最低杀菌浓度在Ag/CuO NPs剂量的广泛范围内，为150至300μg/ml，这有助于杀死（99.99%）所有测试的致病细胞。在120μg/mL的Ag/CuO NPs和100μg/mL（80.43±0.59）下，对大肠杆菌的相对抑制活性（%）最高，分别为81.45±1.39，其次是80μg/mL（72.33±0.82）。此外，在120μg/mL的Ag/CuO NPs下，对真菌细胞和革兰氏阳性菌的相对抑制活性（%）最低，分别为52.17±1.49和53.42±1.71。
