Tamilmani Hemaanhini, Kiran Srinivas B, Suresh Nidhita, Saranya K
Periodontology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Sep 4;16(9):e68644. doi: 10.7759/cureus.68644. eCollection 2024 Sep.
Introduction Modern dentistry prioritizes aesthetic outcomes, making root coverage for gingival recession a key focus. Various approaches, including autologous grafts, address this issue, yet no substitute matches the properties of autogenous connective tissue grafts. The innovative collagen-chitosan-bioglass scaffold presents a promising solution, surpassing the limitations of the traditional methods. This scaffold blends the advantages of collagen with chitosan's antibacterial and regenerative properties, enhanced by bioglass, which promotes tissue healing through angiogenesis. It was evaluated for its physicochemical characteristics, as well as antioxidative and anti-inflammatory properties, making it a promising solution for soft tissue management in dentistry. Materials and methods Chitosan, collagen, and bioglass were combined into a scaffold through the lyophilization process (freeze-drying). Chitosan was sourced from shrimp, collagen from bovine, and the bioglass 1% comprised 58% tetra-ethyl ortho silicate, 33% calcium silicate, and phosphorous pentoxide. After the scaffold was created, it was subjected to physicochemical characterization via scanning electron microscopic and infrared spectroscopic analysis. Its anti-inflammatory and antioxidant properties were evaluated using DPPH (2,2-diphenyl -1-picrylhydrazyl) assay and by measuring the scaffold's radical scavenging activity. Results This study employed infrared spectroscopy and scanning electron microscopy techniques to analyze the sample components and their morphology. The infrared (attenuated total reflection) analysis revealed various elements confirming the presence of all the biomaterials required to fabricate the scaffold. Scanning electron microscope imaging displayed a folded-like morphology with a porous structure. The protein denaturation inhibition increased from 25% at 50 μg of scaffold weight to 45% at 200 μg of scaffold weight. Similarly, the antioxidant activity increased, with values rising from 23% at 50μg to 35% at 200μg of scaffold weight. Conclusion The fabricated collagen-chitosan-bioglass scaffold demonstrates promising antioxidant and anti-inflammatory properties. These findings suggest that this scaffold holds significant potential as a viable substitute for soft tissue augmentation.
引言 现代牙科将美学效果置于优先地位，牙龈退缩的牙根覆盖成为关键关注点。包括自体移植在内的各种方法都致力于解决这一问题，但没有任何替代品能与自体结缔组织移植物的特性相媲美。创新的胶原蛋白 - 壳聚糖 - 生物玻璃支架提供了一个有前景的解决方案，克服了传统方法的局限性。这种支架融合了胶原蛋白的优势与壳聚糖的抗菌和再生特性，并通过生物玻璃得到增强，生物玻璃可通过血管生成促进组织愈合。对其物理化学特性以及抗氧化和抗炎特性进行了评估，使其成为牙科软组织管理的一个有前景的解决方案。
材料与方法 壳聚糖、胶原蛋白和生物玻璃通过冻干过程（冷冻干燥）组合成支架。壳聚糖来源于虾，胶原蛋白来源于牛，1%的生物玻璃由58%的正硅酸四乙酯、33%的硅酸钙和五氧化二磷组成。支架制成后，通过扫描电子显微镜和红外光谱分析对其进行物理化学表征。使用DPPH（2,2 - 二苯基 - 1 - 苦基肼）测定法并测量支架的自由基清除活性来评估其抗炎和抗氧化特性。
结果 本研究采用红外光谱和扫描电子显微镜技术分析样品成分及其形态。红外（衰减全反射）分析揭示了各种元素，证实了制造支架所需的所有生物材料的存在。扫描电子显微镜成像显示出具有多孔结构的折叠状形态。蛋白质变性抑制率从支架重量为50μg时的25%增加到200μg时的45%。同样，抗氧化活性增加，值从50μg时的23%上升到200μg时的35%。
结论 制备的胶原蛋白 - 壳聚糖 - 生物玻璃支架表现出有前景的抗氧化和抗炎特性。这些发现表明，这种支架作为软组织增强的可行替代品具有巨大潜力。
